Evidence mapPaperPMID 41555628Full record

ArticlePhysiological reports2026

Effects of endurance training on skeletal muscle mitochondrial respiration in Siberian huskies and Alaskan huskies.

Silje Sælen-Helgesson, Anne Dragøy Hafstad, Trine Lund, Ingebjørg Helena Nymo, Chiara Ciccone, Shona Hiedi Wood, Lars P Folkow, Monica Alterskjær Sundset

Abstract read
In one paragraph

Article in Physiological reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Silje Sælen-HelgessonDepartment of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.ORCID 0009-0009-6847-0360
Anne Dragøy HafstadCardiovascular Research Group, Department of Medical Biology, Faculty of Health Science, UiT The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0003-0711-9736
Trine LundCardiovascular Research Group, Department of Medical Biology, Faculty of Health Science, UiT The Arctic University of Norway, Tromsø, Norway.ORCID 0009-0008-6894-1890
Ingebjørg Helena NymoDepartment of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0002-4844-8344
Chiara CicconeDepartment of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0002-0749-6198
Shona Hiedi WoodDepartment of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0002-8273-4045
Lars P FolkowDepartment of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0002-6580-9156
Monica Alterskjær SundsetDepartment of Arctic and Marine Biology, UiT The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0002-4850-262X

Funding

Troms Forskningsstiftelse (TFS) IS3_17_SWTroms Forskningsstiftelse (TFS) TFS2016SW
6 · The paper itself

Abstract

Siberian huskies (SH) and Alaskan huskies (AH), sharing ancestry with ancient sled dogs, were hypothesized to achieve similar skeletal muscle (SM) mitochondrial respiration capacities and densities through endurance training. High-resolution respirometry of SM biopsies from SH and AH during off-season (5 SH, 4 AH) and racing-season (5 SH, 7 AH) revealed a striking increase in mass-specific succinate-linked mitochondrial complex II (CII) activity during racing-season, in both SH (+75%) and AH (+129%). These increases were accompanied by increased protein content in SM for both SH (+37%) and AH (+56%). Elevated CII respiratory capacity can potentially reflect increased fatty acid utilization. NADH-linked complex I (CI) respiration increased significantly only in AH (+35%), which also, unlike SH, exhibited significantly elevated citrate synthase activity (+270%). Both groups showed reduced protein-specific residual oxygen consumption during racing-season (SH: -45%, AH: -48%) and increased reactive oxygen species production. Together, these changes point to more efficient mitochondria with minimized energy loss in raced dogs. A minimally invasive sampling approach was validated, using NSAIDs, local anesthesia, light oral sedation, a micro biopsy gun, and individualized environments to minimize distress. This secured good animal welfare and provided a practical method for field-based or repeated SM biopsies without general anesthesia.

Indexed as

Mitochondria, MuscleMuscle, SkeletalPhysical Conditioning, AnimalPhysical EnduranceAnimalsCell RespirationCitrate (si)-SynthaseDogsMaleOxygen ConsumptionCitrate (si)-Synthaseanimal welfareCanis lupus familiariscitrate synthase activity assayendurance traininghigh‐resolution respirometrymitochondrial respiration

Identifiers

PMID41555628
PMCPMC12816770

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.