Evidence map›Paper›PMID 41556345›Full record

ArticleNucleic acids research2026

A noncanonical transcriptional regulation in natural product biosynthesis.

Jinjin Wang, Kehui Wang, Junbo Wang, Shuxin Huang, Baixin Lin, Xiaomeng Wei, Feng Yu, Yongkang Huang, Peiyu Liu, Xueyi Quan and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jinjin WangState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Kehui WangState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Junbo WangState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Shuxin HuangState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Baixin LinState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Xiaomeng WeiState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Feng YuState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Yongkang HuangState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Peiyu LiuState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Xueyi QuanState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Shaoxuan WuState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Zixin DengState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Jianting ZhengState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.ORCID 0000-0003-1250-3556
Delin YouState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.ORCID 0000-0003-1644-9825
Lingxin KongState Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.ORCID 0009-0005-9471-5107

Funding

National Key Research and Development Program of China 2021YFA0909500National Natural Science Foundation of China 32170075National Natural Science Foundation of China 32170077National Natural Science Foundation of China 32570069
6 · The paper itself

Abstract

TenA (transcription enhancement) family proteins are widespread in bacteria, yet their functions in regulating natural product biosynthesis remain largely unexplored. Here, we report that LysR2, a TenA-family protein encoded by the lysolipin I biosynthetic gene cluster, acts as a transcriptional activator. Genetic and biochemical analyses reveal that LysR2 employs a noncanonical mechanism. Lacking a standard DNA-binding domain, it does not bind target promoters directly. Instead, LysR2 activates transcription by specifically binding to the pathway-specific repressor, LysR1. This protein-protein interaction displaces LysR1 from its cognate promoter, thereby derepressing the transcription of the biosynthetic gene cluster. Our work provides the first experimental evidence for a regulatory role of a TenA-family protein and elucidates a unique indirect activation mechanism that broadens the paradigm of transcriptional regulation in bacterial natural product biosynthesis.

Indexed as

Bacterial ProteinsBiological ProductsGene Expression Regulation, BacterialTranscriptional ActivationTranscription FactorsMultigene FamilyPromoter Regions, GeneticProtein BindingRepressor ProteinsTranscription, GeneticBacterial ProteinsBiological ProductsLysR protein, BacteriaRepressor ProteinsTranscription Factors

Identifiers

PMID41556345
PMCPMC12817078

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.