Evidence map›Paper›PMID 41559548›Full record

ArticleBMC microbiology2026

Isolation and production of newly oligosporogenic polyvalent Bacillus thuringiensis mutants for agricultural pest control.

Sameh Sellami, Fatma El Abed, Zayneb Gouader, Mouna Kriaa, Wafa Jallouli, Souad Rouis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sameh SellamiLaboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", Sfax, 3018, Tunisia. sellamisameh@gmail.com.
Fatma El AbedLaboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", Sfax, 3018, Tunisia.
Zayneb GouaderLaboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", Sfax, 3018, Tunisia.
Mouna KriaaLaboratory of Microorganisms and Biomolecules, Center of Biotechnology of Sfax, University of Sfax, Sfax, Tunisia.
Wafa JallouliLaboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", Sfax, 3018, Tunisia.
Souad RouisLaboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box: "1177", Sfax, 3018, Tunisia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTo develop a new generation of eco-friendly biopesticides, this study used an optimized random mutagenesis approach to generates oligosporogenic mutants from B. thuringiensis var. kurstaki BLB1. Although random mutagenesis is not a novel approach, it remains a practical and efficient strategy providing a simple and accessible alternative.

resultsScreening of over than 600 mutants showed that only 14 clones harbour less rate of spores ranging from 1 ± 0.5 10

conclusionThe study successfully generated oligosporogenic mutants of B. thuringiensis BLB1 with enhanced delta-endotoxin and enzyme production while reducing spore formation. The S6 mutant showed the highest bioactivity, and its production was further increased in a cost-effective way using date waste as a culture medium, highlighting its potential as a useful and eco-friendly biopesticide.

Indexed as

Bacillus thuringiensisPest Control, BiologicalSpores, BacterialAgricultureAnimalsBacillus thuringiensis ToxinsBacterial ProteinsBiological Control AgentsEndotoxinsHemolysin ProteinsMutagenesisMutationBacillus thuringiensis ToxinsBacterial ProteinsBiological Control AgentsEndotoxinsHemolysin ProteinsAgro-waste valorisationEco-friendly biopesticideOligosporogenic mutantPhytopathogensRandom mutagenesisSustainable pest control

Identifiers

PMID41559548
PMCPMC12951920

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.