Evidence map›Paper›PMID 41563607›Full record

ArticleInflammation2026

GTS-21 Alleviates Acute Lung Injury by Enhancing GLP-1 Secretion and Regulating Alveolar Surfactant Proteins via α7nAChR Activation.

Chunli Liu, Yuqi Song, Xinghan Tian, Hongkun Quan, Weikun Tian, Niitiggya Taneja, Guirong Wang, Qinghe Meng, Robert N Cooney

Abstract read
In one paragraph

Article in Inflammation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chunli Liu *Intensive Care Unit, Shandong Public Health Clinical Center, Shandong University, Shandong, China.
Yuqi Song *Departments of Surgery, State University of New York (SUNY), Upstate Medical University, 750 E Adams St., Suite 8141, Syracuse, NY, USA.
Xinghan TianDepartments of Surgery, State University of New York (SUNY), Upstate Medical University, 750 E Adams St., Suite 8141, Syracuse, NY, USA.
Hongkun QuanDepartments of Surgery, State University of New York (SUNY), Upstate Medical University, 750 E Adams St., Suite 8141, Syracuse, NY, USA.
Weikun TianDepartments of Surgery, State University of New York (SUNY), Upstate Medical University, 750 E Adams St., Suite 8141, Syracuse, NY, USA.
Niitiggya TanejaDepartments of Surgery, State University of New York (SUNY), Upstate Medical University, 750 E Adams St., Suite 8141, Syracuse, NY, USA.
Guirong WangDepartments of Surgery, State University of New York (SUNY), Upstate Medical University, 750 E Adams St., Suite 8141, Syracuse, NY, USA.
Qinghe MengDepartments of Surgery, State University of New York (SUNY), Upstate Medical University, 750 E Adams St., Suite 8141, Syracuse, NY, USA. mengq@upstate.edu.
Robert N CooneyDepartments of Surgery, State University of New York (SUNY), Upstate Medical University, 750 E Adams St., Suite 8141, Syracuse, NY, USA. cooneyr@upstate.edu.

Funding

Shandong Medical and Health Technology Development Plan 2019WS529
6 · The paper itself

Abstract

GTS-21, a selective α7-nicotinic acetylcholine receptor (α7nAChR) agonist, exhibits significant anti-inflammatory effects in acute lung injury (ALI), but its mechanisms remain incompletely understood. We hypothesize that its protective effects involve the regulation of Glucagon-like peptide-1 (GLP-1) and pulmonary surfactant protein (SP). P. aeruginosa-induced pneumonia (2.5 × 10⁵ CFU/mouse) and LPS-induced ALI (2.5 mg/kg) models via intratracheal instillation (IT) were used in WT, α7nAChR KO and GLP-1R KO mice. Lung injury was assessed via histopathology, cytokine quantification (IL-6, HMGB1), alveolar–capillary barrier injury, immune cell infiltration, circulating GLP-1, and SP expression in bronchoalveolar lavage fluid (BALF). GTS-21 significantly reduced mortality in WT mice with P. aeruginosa pneumonia, while α7nAChR KO mice showed increased mortality. GTS-21 also improved survival, attenuated lung injury, decreased leukocyte infiltration, and reduced HMGB1/protein concentration in BALF. In murine lipopolysaccharide (LPS)-induced ALI, GTS-21 improved histopathology and alveolar–capillary barrier integrity, lowered IL-6 and HMGB1, and enhanced GLP-1 elevation in WT mice but not α7nAChR KO mice. Furthermore, LPS suppressed SP-A and SP-D in BALF, which GTS-21 restored in WT mice. To confirm the role of GLP-1 in GTS-21-mediated lung protection, we examined inflammation in GLP-1R KO mice following LPS challenge. Anti-inflammatory effects of GTS-21 were blunted in these mice, suggesting a GLP-1-dependent mechanism. GTS-21 protects against bacterial pneumonia and ALI through α7nAChR activation, GLP-1 modulation, and SP regulation. These findings provide evidence that the protective effects of GTS-21 on ALI are mediated in part by GLP-1 and pulmonary surfactant.

Indexed as

Acute Lung Injuryalpha7 Nicotinic Acetylcholine ReceptorBenzylidene CompoundsGlucagon-Like Peptide 1Pulmonary Surfactant-Associated ProteinsPyridinesAnimalsBronchoalveolar Lavage FluidLipopolysaccharidesMaleMiceMice, Inbred C57BLMice, Knockout3-(2,4-dimethoxybenzylidene)anabaseinealpha7 Nicotinic Acetylcholine ReceptorBenzylidene CompoundsGlucagon-Like Peptide 1LipopolysaccharidesPulmonary Surfactant-Associated ProteinsPyridinesAcute lung injury (ALI)Glucagon-like peptide-1 (GLP-1)GTS-21Pseudomonas aeruginosa-induced pneumoniaSurfactant protein A/D (SP-A/D)Α7-nicotinic acetylcholine receptor (α7nAChR)

Identifiers

PMID41563607
PMCPMC12883516

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.