Evidence map›Paper›PMID 41565461›Full record

ArticleNeuropathology : official journal of the Japanese Society of Neuropathology2026

Immunohistochemical Analysis of Tom20 in Choroid Plexus Epithelial Cells From Elderly Brains With Neurodegenerative Diseases.

Ryuta Murakami, Yoichi Chiba, Yumi Miyai, Koichi Matsumoto, Keiji Wakamatsu, Yuko Saito, Manato Hara, Shigeo Murayama, Masaki Ueno

Abstract read
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In one paragraph

Article in Neuropathology : official journal of the Japanese Society of Neuropathology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ryuta MurakamiDepartment of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Japan.
Yoichi ChibaDepartment of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Japan.
Yumi MiyaiDepartment of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Japan.
Koichi MatsumotoDepartment of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Japan.
Keiji WakamatsuDepartment of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Japan.
Yuko SaitoDepartment of Neuropathology (Brain Bank for Aging Research), Tokyo Metropolitan Institute of Geriatrics and Gerontology, Tokyo, Japan.
Manato HaraDepartment of Neuropathology (Brain Bank for Aging Research), Tokyo Metropolitan Institute of Geriatrics and Gerontology, Tokyo, Japan.
Shigeo MurayamaDepartment of Neuropathology (Brain Bank for Aging Research), Tokyo Metropolitan Institute of Geriatrics and Gerontology, Tokyo, Japan.
Masaki UenoDepartment of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Miki-cho, Kita-gun, Japan.ORCID https://orcid.org/0000-0002-6592-3573

Funding

Integrated Research Initiative for Living Well with Dementia (IRIDE) of the Tokyo Metropolitan Institute for Geriatrics and GerontologyJapan Agency for Medical Research and Development JP21wm0425019Japan Agency for Medical Research and Development JP24dk0207074h0001Japan Society for the Promotion of Science 22H04923Japan Society for the Promotion of Science 23K10827Japan Society for the Promotion of Science 24K10554Japan Society for the Promotion of Science 24K17847Ministry of Health, Labour and Welfare JPMH23FC1008
6 · The paper itself

Abstract

The choroid plexus (CP) contributes to cerebrospinal fluid (CSF) production and regulation of circadian rhythm. Recently, many neuroimaging studies have reported enlarged CP volumes in patients with neurodegenerative diseases, including Alzheimer's disease (AD). While previous morphometric analysis revealed age-related enlargement of CP epithelial cells, the metabolic significance of such morphological changes including eosinophilic enlarged changes remains unclear. In this study, we investigated mitochondrial alterations in CP epithelial cells using immunohistochemistry for Tom20, a marker of the mitochondrial outer membrane. Temporal lobes, including CP, in patients with AD, vascular dementia, Parkinson's disease, and multiple system atrophy, and neuropathologically unremarkable controls were examined. Quantitative image analysis was performed using AI-assisted segmentation (cellpose) and Python-based processing (OpenCV, NumPy, and Pandas). Cell area and the proportion of Tom20-positive pixels (brown ratio) were measured in 13 090 epithelial cells from 20 cases, and data were analyzed using Spearman's rank correlation. The brown ratio was positively correlated with cell area. Enlarged epithelial cells frequently exhibited strong Tom20 immunoreactivity. These findings suggest that cellular enlargement in the CP epithelium is accompanied by increased mitochondrial content, highlighting a potential metabolic response in neurodegenerative brains.

Indexed as

Choroid PlexusEpithelial CellsNeurodegenerative DiseasesAgedAged, 80 and overFemaleHumansImmunohistochemistryMaleMitochondriaMitochondrial Precursor Protein Import Complex ProteinsMitochondrial Precursor Protein Import Complex ProteinsTOMM20 protein, humanAI‐assisted image analysischoroid plexusimmunohistochemistrymitochondrianeurodegenerationTom20

Identifiers

PMID41565461
PMCPMC12823287

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.