Cellular multipoint adaptive technology for two-photon mesoscope.

Significance: In mesoscopic imaging research in neuroscience, achieving high spatial resolution optical imaging across the entire field of view (FOV) remains critical. This directly determines whether researchers can precisely analyze the large-scale dynamic activities of neural circuits at the single-cell or even subcellular level. Consistent optical quality throughout the entire imaging FOV is essential to accurately capture the spatiotemporal patterns of neural activity across brain regions, thereby providing a powerful tool for understanding the circuit mechanisms underlying cognition, behavior, and disease at cellular and subcellular resolution Aim: This study aims to develop a technology that extends the imaging FOV in a two-photon mesoscope while enhancing the optical quality across the entire FOV Approach: This study introduces an innovative approach that combines block scanning with adaptive optical (AO) correction through a bioinspired honeycomb-based cellular multipoint adaptive technology (CMAT) to achieve mesoscopic two-photon imaging. This system enables unprecedented large-FOV, high-resolution imaging by dividing an Results: Comprehensive evaluation using standard samples and transgenic mouse models demonstrated that the CMAT significantly enhances the imaging performance of the two-photon mesoscope. This technique extends the effective two-photon imaging FOV from Conclusion: CMAT significantly extends the effective FOV and enhances the optical quality of the two-photon mesoscope system.