ArticleFrontiers in bioengineering and biotechnology2025
Systematic evaluation of pigment-based whole-cell lead biosensors: challenges in genetic circuit engineering and critical considerations for background noise control.
Article in Frontiers in bioengineering and biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Despite advances in whole-cell biosensors for Pb(II) detection, background leakage and limited dynamic range hinder field deployment. This study systematically evaluates four diverse Methods: pbr operons from Results: The pLVPK-derived pKp-DV construct (PbrR.Kp) achieved optimal performance: LOD of 0.0008 μM, dynamic range 0.0061-50 μM, and high Pb(II) specificity. Transcription terminator insertion further reduced LOD to 0.0002 μM and background noise by 60%. However, regulator dosage and hybrid circuits showed minimal improvement, indicating limited synergistic benefits. Discussion: PbrR.Kp selection and transcriptional insulation are key strategies for reducing leakage. Hybrid circuits require more sophisticated designs. Future work should focus on chromosomal integration and environmental matrix validation to ensure the development of robust, field-ready sensors.
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