Rapid Structural Analysis of Natural Products Using MicroED.

Jieye Lin, Orel Paz, Johan Unge, Tamir Gonen

Abstract read

In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Jieye LinDepartment of Biological Chemistry, University of California, Los Angeles, Los Angeles, California, USA.ORCID https://orcid.org/0009-0003-9008-5115

Orel PazDepartment of Biological Chemistry, University of California, Los Angeles, Los Angeles, California, USA.

Johan UngeDepartment of Chemistry, Umeå University, Umeå, Sweden.

Tamir GonenDepartment of Biological Chemistry, University of California, Los Angeles, Los Angeles, California, USA.ORCID https://orcid.org/0000-0002-9254-4069

Funding

TRD4: Design and Engineering of MicroED hardware for nanocrystallization and time resolved studiesP41GM136508 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GONEN, TAMIR, NANNENGA, BRENT · 2020 to 2024

$7.6M

MEDIC-MicroED Imaging Center at UCLARM1GM158451 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Tamir Gonen, Brent Nannenga · 2025 to 2026

$2.7M

Department of Defense MCDC-2202-002Howard Hughes Medical InstituteNIGMS NIH HHS RM1 GM158451NIH HHS P41GM136508NIH HHS RM1GM158451

6 · The paper itself

Abstract

Structural analysis of natural products remains challenging due to their inherent complexity and limited availability. By solving nine chemically diverse natural products of high priority that have resisted reported 3D structures for years, we demonstrate that microcrystal electron diffraction (MicroED) can systematically and feasibly be applied to natural-product structure elucidation on samples that were previously unattainable by other structural methods. In addition, using microgram quantities of material and side-stepping the process to create large single crystals, the success rate is still comparable to other techniques on a general sample using larger amounts of the sample. We suggest that MicroED is not only a necessary technique but also a general and efficient tool for many applications.

Indexed as

Biological ProductsCrystallography, X-RayElectronsBiological Productscrystallographymicrocrystal electron diffraction (MicroED)natural productsstructural analysis

Identifiers

PMID41574732

DOI10.1002/smll.202511875

PMCPMC12980483

What Socratic holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.