Evidence map›Paper›PMID 41574767›Full record

ArticleThe European journal of neuroscience2026

The ER/Golgi Protein FNDC3B Facilitates Climbing Fibre to Purkinje Cell Synapse Elimination in the Developing Mouse Cerebellum.

Céline Louise Mercier, Takaki Watanabe, Yuto Okuno, Kyoko Matsuyama, Kyoko Kushibe, Henry Denny, Taisuke Miyazaki, Miwako Yamasaki, Meiko Kawamura, Manabu Abe and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The European journal of neuroscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Céline Louise MercierDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Takaki WatanabeDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Yuto OkunoDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.ORCID 0009-0006-3151-3599
Kyoko MatsuyamaDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Kyoko KushibeDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Henry DennyDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Taisuke MiyazakiDepartment of Anatomy, Faculty of Medicine, Hokkaido University, Sapporo, Japan.
Miwako YamasakiDepartment of Anatomy, Faculty of Medicine, Hokkaido University, Sapporo, Japan.
Meiko KawamuraDepartment of Animal Model Development, Brain Research Institute, Niigata University, Niigata, Japan.
Manabu AbeDepartment of Animal Model Development, Brain Research Institute, Niigata University, Niigata, Japan.
Kenji SakimuraDepartment of Animal Model Development, Brain Research Institute, Niigata University, Niigata, Japan.
Masahiko WatanabeDepartment of Anatomy, Faculty of Medicine, Hokkaido University, Sapporo, Japan.
Naofumi UesakaDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
Masanobu KanoDepartment of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.ORCID 0000-0002-0725-3292

Funding

Japan Society for the Promotion of Science 18H04012Japan Society for the Promotion of Science 20H05915Japan Society for the Promotion of Science 21H04785
6 · The paper itself

Abstract

Synapse elimination during development is crucial for refining neural circuits by removing excess synapses formed around birth. In the neonatal cerebellum, Purkinje cells (PCs) are initially innervated by multiple climbing fibers (CFs) with similar synaptic strengths. During subsequent postnatal development, a single CF is strengthened and retained, while the other CFs are eliminated. Here, our PC-specific RNAi knockdown (KD) screening revealed that fibronectin type III domain containing 3B (FNDC3B), an endoplasmic reticulum protein, was involved in CF synapse elimination from around postnatal day 9 (P9) in mice. We showed that FNDC3B mRNA was expressed in PCs during CF synapse elimination. In PC-selective FNDC3B conditional knockout (FNDC3B-cKO) mice, CF synapse elimination from P10 was impaired, and the extension of CFs along PC dendrites was reduced at P21. However, these phenotypes were recovered by P40. In contrast, parallel fiber-mediated excitatory synaptic inputs and inhibitory synaptic inputs to PCs were not affected in FNDC3B-cKO mice. These results suggest that FNDC3B facilitates CF synapse elimination during postnatal development, highlighting a new role of FNDC3B in the developing brain.

Indexed as

CerebellumFibronectinsNerve FibersPurkinje CellsSynapsesAnimalsEndoplasmic ReticulumGolgi ApparatusMiceMice, Inbred C57BLMice, KnockoutNeurodevelopmentFibronectinscerebellumclimbing fiberFNDC3Bpostnatal developmentPurkinje cellsynapse elimination

Identifiers

PMID41574767
PMCPMC12828876

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.