Evidence map›Paper›PMID 41576153›Full record

ArticleScience advances2026

MAcro Plant Projection Imaging (MAPPI): An open, scalable platform for whole-plant fluorescence real-time imaging.

Giorgia Tortora, Bianca Maria Orlando Marchesano, Stefano Buratti, Laura Luoni, Matteo Grenzi, Keiko Yoshioka, Valeria Contartese, Alessia Candeo, Alex Costa, Andrea Bassi

Abstract read
In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Giorgia TortoraDepartment of Physics, Politecnico di Milano, Piazza Leonardo da Vinci, 20133 Milan, Italy.ORCID 0000-0003-1395-368X
Bianca Maria Orlando MarchesanoDepartment of Biosciences, Università degli Studi di Milano, Via G. Celoria 26, 20133 Milan, Italy.ORCID 0009-0002-6441-8169
Stefano BurattiDepartment of Biosciences, Università degli Studi di Milano, Via G. Celoria 26, 20133 Milan, Italy.ORCID 0000-0002-7090-8722
Laura LuoniDepartment of Biosciences, Università degli Studi di Milano, Via G. Celoria 26, 20133 Milan, Italy.ORCID 0000-0002-6485-5417
Matteo GrenziDepartment of Biosciences, Università degli Studi di Milano, Via G. Celoria 26, 20133 Milan, Italy.ORCID 0000-0003-2295-0018
Keiko YoshiokaDepartment of Cell and Systems Biology, University of Toronto, Toronto, ON, Canada.ORCID 0000-0002-3797-4277
Valeria ContarteseResearch and Development Department, Green Has Italia S.p.A, Canale, Cuneo, Italy.ORCID 0000-0003-4346-1459
Alessia CandeoDepartment of Physics, Politecnico di Milano, Piazza Leonardo da Vinci, 20133 Milan, Italy.ORCID 0000-0001-9597-3056
Alex CostaDepartment of Biosciences, Università degli Studi di Milano, Via G. Celoria 26, 20133 Milan, Italy.ORCID 0000-0002-2628-1176
Andrea BassiDepartment of Physics, Politecnico di Milano, Piazza Leonardo da Vinci, 20133 Milan, Italy.ORCID 0000-0002-5017-0775

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Understanding how plants perceive and respond to environmental and developmental cues requires tools capable of monitoring molecular signals in vivo, across whole tissues, and in real time. Genetically encoded fluorescent indicators, coupled with fluorescence microscopy, have transformed plant biology, but their application remains largely confined to small model organisms and specialized microscopy instrumentation. Here, we present MAcro Plant Projection Imaging (MAPPI), an open-source, low-cost, and modular fluorescence imaging platform for soil-grown plants beyond the model organism or seedling stage. MAPPI enables wide field-of-view, dual-projection imaging of fluorescent reporters, supporting real-time visualization of systemic signals under near-physiological conditions. We validate MAPPI by tracking calcium and l-glutamate dynamics in adult

Indexed as

NicotianaOptical ImagingCalciumCalcium SignalingGlutamic AcidMicroscopy, FluorescencePlant RootsCalciumGlutamic Acid

Identifiers

PMID41576153
PMCPMC12829558

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.