Evidence map›Paper›PMID 41578374›Full record

ArticleJournal of animal science and biotechnology2026

Impact of zearalenone on quorum sensing signaling molecules and its association with the suppression of ruminal microbial fermentation in a RUSITEC system.

Zuo Wang, Tianyi Ma, Jianhua He, Yu Ge, Qianglin Liu, Xinyi Lan, Lei Liu, Fachun Wan, Weijun Shen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of animal science and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zuo WangYuelushan Laboratory, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Tianyi MaYuelushan Laboratory, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Jianhua HeYuelushan Laboratory, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Yu GeYuelushan Laboratory, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Qianglin LiuYuelushan Laboratory, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Xinyi LanYuelushan Laboratory, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Lei LiuCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China.
Fachun WanYuelushan Laboratory, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China. wanfc@hunau.edu.cn.
Weijun ShenYuelushan Laboratory, College of Animal Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128, People's Republic of China. shenweijun@hunau.edu.cn.

Funding

Hunan Herbivores Industry Technological System HARS-08Hunan Provincial Natural Science Foundation 2024JJ5179Key laboratory for the feed and biology technique of Xinjiang Uygur Autonomous Region XJSLSW-2023001National Natural Science Foundation of China 32302764
6 · The paper itself

Abstract

backgroundZearalenone (ZEN), a common mycotoxin in ruminant diets, could disturb the rumen ecosystem and impair rumen fermentation. Noticeably, ZEN has been shown to reduce the relative abundances of specific bacterial taxa that potentially possess quorum sensing (QS) functions, which are deemed essential for the microbial interactions and adaptations during rumen fermentation. Nonetheless, whether QS communications participate in the responses of rumen microbial fermentation to ZEN remains unknown. Therefore, the present trial was performed to explore the potential roles of QS during the alterations of rumen microbial fermentation by ZEN through a rumen simulation technique (RUSITEC) system, in a replicated 4 × 4 Latin square design.

resultsZEN significantly (P < 0.05) reduced QS signal autoinducer-2 (AI-2), and tended to (P = 0.051) downregulate QS signal C4-homoserine lactone (HSL). ZEN also significantly (P < 0.05) decreased total volatile fatty acid (TVFA), acetate, propionate, isobutyrate, isovalerate, organic matter disappearance (OMD), neutral detergent fiber disappearance (NDFD), and acid detergent fiber disappearance (ADFD) in different manners. The linear discriminant analysis effect size (LEfSe) analysis indicated significantly (P < 0.05) differential enrichments of a series of bacterial taxa such as Butyrivibrio_sp_X503, Rhizobium daejeonense, Hoylesella buccalis, Ezakiella coagulans, Enterococcus cecorum, Ruminococcus_sp_zg-924, Polystyrenella longa, and Methylacidimicrobium fagopyrum across different treatments. The phylogenetic investigation of communities by reconstruction of unobserved states 2 (PICRUSt2) analysis suggested that QS were predicted to be significantly (P < 0.05) affected by ZEN. The metabolomics analysis detected considerable significantly (P < 0.05) differing metabolites and implied that ZEN challenge significantly (P < 0.05) influenced the indole alkaloid biosynthesis, biosynthesis of alkaloids derived from shikimate pathway, and sesquiterpenoid and triterpenoid biosynthesis. Significant (P < 0.05) interconnections of QS molecules with the differential rumen fermentation traits, differential bacterial taxa, and differential metabolites were exhibited by Spearman analysis.

conclusionsZEN negatively affected the QS signals of AI-2 and C4-HSL, which was found to correlate with the fluctuations in specific rumen fermentation characteristics, ruminal bacterial populations, and ruminal metabolisms. These interrelationships implied the potential involvement of QS in the reactions of rumen microbiota to ZEN contamination, and probably contributed to the inhibition of rumen fermentation.

Indexed as

Acyl-homoserine lactonesAutoinducer-2Quorum sensingRumen microbiomeZearalenone

Identifiers

PMID41578374
PMCPMC12831299

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.