Evidence mapPaperPMID 41580599Full record

ArticleClinical proteomics2026

Comprehensive analysis of proteomics and lactylation proteomics in ovarian granulosa cells of patients with polycystic ovary syndrome.

Li Liu, Qian Gao, Jingyu Huang, Yu Qian, Kailu Liu, Yun Tong, Yaqiong Zeng, Yueyi Li, Yunteng Liang, Yanli Hong and 2 more

Abstract read
In one paragraph

Article in Clinical proteomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Li Liu *Department of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Qian Gao *Department of Gynaecology and Obstetric, Yixing Traditional Chinese Medicine Hospital, Yixing, Jiangsu, China.
Jingyu Huang *Department of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Yu QianDepartment of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Kailu LiuDepartment of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Yun TongDepartment of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Yaqiong ZengDepartment of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Yueyi LiDepartment of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Yunteng LiangDepartment of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China.
Yanli HongDepartment of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China. 15951711586@126.com.
Huifang ZhouDepartment of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China. yfy0005@njucm.edu.cn.
Xiaowei NieDepartment of Reproductive Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China. fsyy00636@njucm.edu.cn.

Funding

Provincial Department of Education Postgraduate Innovation Project KYCX24_2245the National Natural Science Foundation of China 82074479Yixing Taodu Light Science and Technology Research Plan 2023SF12
6 · The paper itself

Abstract

backgroundPolycystic ovary syndrome (PCOS) is a complex and heterogeneous metabolic disorder that affects 6-20% of women of reproductive age. However, research on the lactylation-modified proteome in PCOS remains limited.

methodsThis study included 30 patients with PCOS and 30 control subjects, all of whom underwent intracytoplasmic sperm injection or in vitro fertilization-embryo transfer treatments at the fertility center between October 2022 and May 2023. A 4-dimensional label-free proteomic quantitation method was applied to analyze enzymatically digested peptide fragments of granulosa cell proteins. Liquid chromatography-mass spectrometry was used for protein identification and quantification.

resultsBioinformatics analysis of differentially expressed proteins (DEPs) and differentially lactylated proteins identified 1057 DEPs between the two groups. Among these, 478 proteins were upregulated, and 579 were downregulated in the PCOS group. Regarding lactylation modifications, 668 proteins exhibited increased lactylation levels, while 1059 proteins indicated decreased lactylation levels in the PCOS group. Additionally, site-level analysis revealed 1041 upregulated and 2143 downregulated lactylation sites in the PCOS group.

conclusionsThis study provides a comprehensive quantitative overview of proteomic and lactylation-modified proteomic expression profiles in granulosa cells from patients with PCOS, offering novel insights into PCOS research. Further research is needed to clarify the specific roles of protein lactylation in PCOS pathogenesis.

Indexed as

Bioinformatics analysisGranulosa cellsNovel posttranslational modificationPolycystic ovary syndrome

Identifiers

PMID41580599
PMCPMC12922295

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.