ArticleBMC medical genomics2026
Discovery of new MicroRNAs and their mRNA targets in patients with acute ischemic stroke.
Article in BMC medical genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Mechanisms of microRNA export in mammalian cells: From random release to selective secretion.The Journal of biological chemistry · 2026Review
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11 authors.
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Abstract
backgroundIn this study, we applied microarray, bioinformatics, and qRT-PCR techniques to identify miRNAs and their target genes in plasma obtained from acute ischemic stroke patients and matching controls.
methodsMicroarray analyses were performed with 24-h acute ischemic stroke vs. healthy individuals and CV-risk factors matched control group plasma samples. Statistical analysis of gene expression was performed using TAC and R, with a focus on robust methods suitable for the small sample size, and miRNA target prediction was conducted using a previously established in-house wizbionet R package. Top non-coding regulators of ischemia (miR-18a-5p, miR-4467, miR-199a-5p and miR-3135b) and their predicted target genes (ANKRD12, HIF1A, GNAI2, GRIN1) were detected via qRT-PCR.
results146 upregulated and 258 downregulated differentially expressed RNAs were detected by microarray analysis. Using the multiMiR R package for target prediction, 67 upregulated and 125 downregulated mRNAs were mapped. Functional enrichment analysis revealed that upregulated miRNAs were associated with pathways like BDNF and IL-2 signaling, while downregulated miRNAs were linked to neurodevelopmental and NGF pathways. MiR-18a-5p and miR-199a-5p were significantly elevated in stroke patients at both day 1 and day 7 compared to healthy individuals and CV-matched controls (p < 0.05 for all). miR-4467 was lower at day 1 versus both controls (p < 0.05) but markedly increased by day 7 (p < 0.001), remaining higher than in controls (p < 0.05). miR-3135b showed persistent downregulation at both time points (p < 0.05). ROC analysis confirmed diagnostic value for all miRNAs, with the highest AUC for miR-199a-5p (0.89, 95% CI: 0.81–0.97, p < 0.001), followed by miR-3135b (0.88), miR-4467 (0.80), and miR-18a-5p (0.73; p = 0.002). For potential role in dynamic post-stroke molecular responses perspective, utility, miR-4467 increased significantly during hospitalization (p < 0.001). GNAI2 mRNA was significantly elevated at day 1 versus controls (p < 0.05). ANKRD12 was consistently lower at both day 1 and day 7 compared to controls (p < 0.05). GRIN1 was reduced at admission (p = 0.006) but normalized by day 7 (p > 0.05). ROC analysis showed diagnostic significance for ANKRD12, GNAI2, and GRIN1 (AUC = 0.683, 0.698, 0.693).
conclusionOur integrated miRNA/mRNA analysis identified distinct molecular signatures in acute ischemic stroke, with 146 upregulated and 258 downregulated RNAs, implicating key neuroinflammatory and neuroprotective pathways, including BDNF, IL-2, and NGF signaling. Among the validated candidates, miR-199a-5p, miR-3135b, miR-4467, and miR-18a-5p demonstrated diagnostic potential, while miR-4467, together with GNAI2 and HIF1A, showed post-stroke dynamic relevance, reflecting early transcriptomic adaptations following ischemic injury.
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