Evidence map›Paper›PMID 41588920›Full record

ArticleJournal of cellular and molecular medicine2026

Single-Cell RNA Sequencing Analysis Reveals Correlation Between Immune Cell Composition and Gene Expression in Cervical Cancer.

Changchang Huang, Guosha Pang, Xiaolin Lang, Jingjing Zhang, Fen Zhao

Abstract read
In one paragraph

Article in Journal of cellular and molecular medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Changchang HuangDepartment of Gynecology, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Guosha PangDepartment of Gynecology, Xingqiao Community Health Service Center, Hangzhou, Zhejiang, China.
Xiaolin LangDepartment of Gynecology, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Jingjing ZhangDepartment of Gynecology, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.
Fen ZhaoDepartment of Gynecology, First People's Hospital of Linping District, Hangzhou, Zhejiang, China.ORCID https://orcid.org/0000-0002-0965-9712

Funding

Hangzhou Science and Technology Plan Guiding Project 2022ZDSJ0675The Zhejiang Provincial Medical and Health Science and Technology Project 2025KY196
6 · The paper itself

Abstract

Cervical cancer has become a glaring concern for women's health globally. The use of single-cell RNA sequencing (scRNA-seq) contributes to a comprehensive understanding of cellular heterogeneity and the immune cell landscape in the TME of cervical cancer. This study is to investigate the distribution pattern of immune cell subsets and their correlation with some gene expression based on single-cell RNA sequencing (scRNA-seq) data in patients with cervical cancer. We collected cervical cancer single-cell RNA sequencing data and explored the quality of the data using the violin plots, scatter plots, variance plots and elbow plots, as well as a search for highly variable genes. We clustered cells with UMAP and t-SNE clustering analyses and then labelled cell populations via flow cytometry and immunohistochemistry. We also analysed the biological functions of critical genes using GO enrichment analysis, and the expression patterns of individual genes at the single-cell level. Lastly, we calculated the shift of immune cell proportion and explored the relationship between key genes like TNFRSF18 and immune cell subgroups. We identified 12 unique cell populations in cervical cancer samples and stained positive for epithelial cells, T cells and macrophages. Functional enrichment analysis revealed the gene expression pattern associated with multiple biological processes and molecular interactions in the tumour microenvironment. Certain genes, such as 16 FOXP3 and CD8A, displayed different expression patterns across the immune cell subsets. Additionally, the expression of TNFRSF18 was directly related to the proportions of most of the immune cells and inversely related to a few T and B lymphocyte subsets. This study offers a comprehensive landscape of immune cell proportions within the cervical cancer TME and uncovers a complexity in the relationships between gene expression and tumour-infiltrating immune cell subsets. These results will provide valuable clues for the study of the immune microenvironment in cervical cancer and will shed some light into novel therapeutic approaches.

Indexed as

Gene Expression Regulation, NeoplasticSequence Analysis, RNASingle-Cell AnalysisUterine Cervical NeoplasmsFemaleGene Expression ProfilingHumansMacrophagesSingle-Cell Gene Expression AnalysisTumor Microenvironmentcervical cancergene expressionimmune cellssingle‐cell RNA sequencingtumour microenvironment

Identifiers

PMID41588920
PMCPMC12835611

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.