ArticleAdvanced biology2026
Cryopreservation of Anopheles gambiae Larvae.
Article in Advanced biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
3 citing papers in PubMed.
- Cryopreservation and other preservation approaches for animal models workshop session I: Invertebrate models in biomedical research.Cryobiology · 2026Article
- Whole testicular organ and seminal cryopreservation in the malarial mosquito Anopheles gambiae (Diptera: Culicidae).Journal of medical entomology · 2026Article
- Cryopreservation of Anopheles gambiae Larvae.Advanced biology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Mosquitoes remain a significant global health concern due to their role in transmitting deadly diseases including malaria, dengue, and yellow fever. Recent advances in genetic modification technologies have enabled researchers to better study disease transmission and is an emerging approach for vector control. However, a major bottleneck is the need for continuous rearing of each new strain, an intensive process requiring substantial manual effort and space with inherent risks of cross-contamination, genetic drift, and catastrophic loss. Cryopreservation offers a potential solution, where strains could be stored indefinitely and revived on an as-needed basis. Here, we report a cryopreservation protocol that results in the recovery of viable Anopheles gambiae larvae. After thawing, 82% of larvae showed signs of viability, including wriggling and mouth brush movements. Approximately 15% further exhibited coordinated swimming behavior. Although post-thaw survival was limited to 24 h, this study provides the first evidence that mosquito larvae can survive cryopreservation, representing a key milestone toward long-term storage of mosquito lines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.