Evidence map›Paper›PMID 41592176›Full record

ArticleJournal of the American Chemical Society2026

Nanobody-Based Bioconjugates as Potent and Broadly Active Inhibitors of HIV Entry.

Shubhra Jyoti Saha, Rashmi Kumariya, Phoenix A Davis, Emily Tourtellott, Nicole Doria-Rose, Carole A Bewley, Ross W Cheloha

Abstract read
In one paragraph

Article in Journal of the American Chemical Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Shubhra Jyoti SahaLaboratory of Bioorganic Chemistry; National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States.
Rashmi KumariyaLaboratory of Bioorganic Chemistry; National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States.ORCID 0000-0001-6498-7447
Phoenix A DavisLaboratory of Bioorganic Chemistry; National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States.
Emily TourtellottVirology Laboratory; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States.
Nicole Doria-RoseVirology Laboratory; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States.
Carole A BewleyLaboratory of Bioorganic Chemistry; National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States.
Ross W ChelohaLaboratory of Bioorganic Chemistry; National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, United States.ORCID 0000-0001-9871-8333

Funding

Inhibitors and Probes of HIV-1 Cell FusionZIADK032103 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI BEWLEY, CAROLE · 2009 to 2025
$12.5M
New tools from chemistry and antibodies to address receptor functionZIADK075157 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI CHELOHA, ROSS · 2021 to 2025
$6.6M
Quantitative Immunological Assays to Assess HIV Antibodies and VaccinesZICAI005010 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI PIERSON, THEODORE · 2019 to 2025
$5.6M
Development of new antibody-fusion inhibitor conjugates to block HIV infectionZIADK075184 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI CHELOHA, ROSS · 2024 to 2025
$2.1M
Antibody-protein conjugates as inhibitors of HIV/AIDSZIADK075187 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI BEWLEY, CAROLE · 2024 to 2025
$1.1M
Intramural NIH HHS ZIA DK032103Intramural NIH HHS ZIA DK075157Intramural NIH HHS ZIA DK075184Intramural NIH HHS ZIA DK075187Intramural NIH HHS ZIC AI005010
6 · The paper itself

Abstract

We describe a semisynthetic strategy that unites the versatility of chemical peptide synthesis with the high affinity and specificity of antibody fragments to create conjugates with remarkably potent anti-HIV activity. Using site-selective enzymatic ligation and click chemistry, we assembled nanobody-fusion inhibitor peptide (Nb-FI) conjugates that link synthetic HIV-1 fusion inhibitor peptides to nanobodies targeting host receptors. This modular approach enables variation of Nb-FI linkage geometry, including C-to-C terminal topologies inaccessible by genetic fusion. Selected semisynthetic conjugates exhibit antiviral potencies up to 10,000-fold better than comparator peptides and broad-spectrum activity across diverse HIV strains. Lead semisynthetic conjugates also outperform analogous genetic fusions. These findings underscore the power of combining chemical synthesis, protein engineering, and antibody delivery to leverage proximity effects in blocking viral infection.

Indexed as

Anti-HIV AgentsHIV-1HIV Fusion InhibitorsPeptidesSingle-Domain AntibodiesVirus InternalizationHumansAnti-HIV AgentsHIV Fusion InhibitorsPeptidesSingle-Domain Antibodies

Identifiers

PMID41592176
PMCPMC12903845

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.