Evidence map›Paper›PMID 41595455›Full record

ArticleGenes2025

Embryonic Thermal Manipulation Affects Neurodevelopment and Induces Heat Tolerance in Layers.

Zixuan Fan, Yuchen Jie, Bowen Niu, Xinyu Wu, Xingying Chen, Junying Li, Li-Wa Shao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zixuan FanFrontier Science Center for Molecular Design Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing 100193, China.
Yuchen JieFrontier Science Center for Molecular Design Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing 100193, China.
Bowen NiuFrontier Science Center for Molecular Design Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing 100193, China.
Xinyu WuFrontier Science Center for Molecular Design Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing 100193, China.
Xingying ChenFrontier Science Center for Molecular Design Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing 100193, China.
Junying LiFrontier Science Center for Molecular Design Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing 100193, China.
Li-Wa ShaoFrontier Science Center for Molecular Design Breeding, State Key Laboratory of Animal Biotech Breeding, China Agricultural University, Beijing 100193, China.ORCID 0009-0007-5347-9368

Funding

China Agriculture Research Systems CARS-40National Key Research and Development Program of China 2022YFF1000204
6 · The paper itself

Abstract

BACKGROUND/

objectivesThe poultry industry faces severe heat-stress challenges that threaten both economic sustainability and animal welfare. Embryonic thermal manipulation (ETM) has been proposed as a thermal programming strategy to enhance chick heat tolerance, yet its efficacy in layers requires verification, and its effects on growth performance and neurodevelopment remain unclear.

methodsWhite Leghorn embryos at embryonic days 13 to 18 (ED 13-18) were exposed to 39.5 °C (ETM). Hatch traits and thermotolerance were recorded, and morphometric and histopathological analyses were performed on brain sections. Transcriptome profiling of the whole brains and hypothalami was conducted to identify differentially expressed genes (DEGs). Representative pathway genes responsive to ETM were validated by RT-qPCR.

resultsETM reduced hatchability, increased deformity rate, and decreased hatch weight and daily weight gain. During a 37.5 °C challenge, ETM chicks exhibited delayed panting and lower cloacal temperature. Histopathology revealed impaired neuronal development and myelination. Transcriptomic analysis of ED18 whole brains showed DEGs enriched in neurodevelopment, stimulus response, and homeostasis pathways. RT-qPCR confirmed hypothalamic sensitivity to ETM: up-regulation of heat-shock gene

conclusionsETM improves heat tolerance in layers but compromises hatching performance and brain development, with widespread perturbation of hypothalamic stress responses and neurodevelopmental gene networks. These findings elucidate the mechanisms underlying ETM and provide a reference for enhancing thermotolerance in poultry.

Indexed as

ChickensThermotoleranceAnimalsBrainChick EmbryoGene Expression ProfilingGene Expression Regulation, DevelopmentalHeat-Shock ResponseHot TemperatureNeurodevelopmentTranscriptomeembryonic thermal manipulationheat tolerancelayermolecular regulatory networkneurodevelopment

Identifiers

PMID41595455
PMCPMC12840955

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.