Next-Generation Strategies for Controlling Foodborne Pathogens: Precision Antimicrobials, Biofilm Disruption, and Emerging Molecular Interventions.

Foodborne diseases remain a major global challenge because pathogenic microorganisms persist in food systems, often protected by biofilms and increasing resistance to conventional chemical preservatives and sanitizers. Control strategies that were effective in the past are becoming less reliable in complex processing environments, creating a need for more precise and adaptable food-safety approaches. This review examines emerging technologies that shift food safety from broad, reactive control toward targeted, data-driven intervention. Biological tools, including bacteriophages, phage-derived enzymes, bacteriocins, quorum-sensing inhibitors, and gene-guided antimicrobial systems, are discussed for their capacity to selectively control specific pathogens while limiting unintended effects on beneficial microbiota. The review also addresses nano-enabled strategies that improve antimicrobial stability, delivery, and performance, along with plant-derived and microbial bioactive compounds that support clean-label and sustainable preservation. In parallel, advances in anti-biofilm surface engineering, such as nano-textured, contact-active, and responsive materials, are examined as preventive measures to reduce microbial attachment and persistence on food-contact surfaces. Beyond individual interventions, this review emphasizes integration within coordinated multi-hurdle systems supported by real-time monitoring and predictive analytics. Emerging digital frameworks, including digital twins of food-processing lines, are highlighted as tools to link detection, risk prediction, and targeted control. Finally, remaining knowledge gaps, regulatory challenges, and research priorities are identified, highlighting the need for realistic testing, long-term safety evaluation, standardized validation, and collaborative efforts to translate precision food-safety technologies into dependable real-world applications.