Evidence mapPaperPMID 41597200Full record

ArticleCells2026

Establishment of Patient-Derived Organoids from Hepatocellular Carcinoma: Preliminary Data on Yield, Histopathological Concordance, and Methodological Challenges.

Oriana Lo Re, Christian Corti, Lucia Cerrito, Eleonora Cesari, Elisabetta Creta, Flavio De Maio, Alessia Di Prima, Vincenzo Facciuto, Clelia Ferraro, Eleonora Huqi and 14 more

Abstract read
In one paragraph

Article in Cells, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Oriana Lo ReIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.ORCID 0000-0002-4798-6802
Christian CortiGSTep Organoids Research Core Facility, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.ORCID 0009-0008-2804-3973
Lucia CerritoLiver Unit, CeMAD Centro Malattie dell'Apparato Digerente, Medicina Interna e Gastroenterologia, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.ORCID 0000-0001-6837-7582
Eleonora CesariGSTep Organoids Research Core Facility, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.
Elisabetta CretaLiver Unit, CeMAD Centro Malattie dell'Apparato Digerente, Medicina Interna e Gastroenterologia, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.ORCID 0009-0002-4716-6000
Flavio De MaioDepartment of Laboratory and Hematological Sciences, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.
Alessia Di PrimaIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.
Vincenzo FacciutoLiver Unit, CeMAD Centro Malattie dell'Apparato Digerente, Medicina Interna e Gastroenterologia, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.
Clelia FerraroIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.
Eleonora HuqiCeMAD Translational Research Laboratories, Digestive Disease Center (CeMAD), Department of Medical and Surgical Sciences, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.
Rosa LiottaIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.
Margot Lo PintoIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.ORCID 0009-0003-0394-9414
Duilio PaganoIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.ORCID 0000-0003-3987-9262
Riccardo PerrieraIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.
Valentina PetitoCeMAD Translational Research Laboratories, Digestive Disease Center (CeMAD), Department of Medical and Surgical Sciences, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.ORCID 0000-0003-4396-1613
Giulia SantarelliDepartment of Laboratory and Hematological Sciences, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.
Francesco SantopaoloLiver Unit, CeMAD Centro Malattie dell'Apparato Digerente, Medicina Interna e Gastroenterologia, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.
Leonardo StellaLiver Unit, CeMAD Centro Malattie dell'Apparato Digerente, Medicina Interna e Gastroenterologia, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.ORCID 0000-0002-3431-0803
Floriana TortomasiIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.ORCID 0000-0001-5035-3435
Claudio SetteGSTep Organoids Research Core Facility, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.ORCID 0000-0003-2864-8266
Salvatore GruttadauriaIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.ORCID 0000-0002-9684-8035
Felice GiulianteHepatobiliary Surgery Unit, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.
Giovanni ZitoIstituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione ISMETT IRCCS, 90127 Palermo, Italy.ORCID 0000-0002-0482-6729
Francesca Romana PonzianiLiver Unit, CeMAD Centro Malattie dell'Apparato Digerente, Medicina Interna e Gastroenterologia, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Rome, Italy.ORCID 0000-0002-5924-6238

Funding

European Union PNRR-MCNT2-2023-12378263
6 · The paper itself

Abstract

Patient-derived organoids (PDOs) have emerged as promising preclinical models for studying tumor biology and testing therapeutic strategies in oncology. These three-dimensional culture systems retain key histological, genetic, and functional characteristics of the original tumors, offering a unique opportunity to advance personalized medicine approaches in liver cancer. In this study, we present the methodological framework and preliminary findings of a prospective study aimed at generating and characterizing PDOs from patients with hepatocellular carcinoma (HCC) undergoing surgical resection. Tumor specimens were processed using an optimized protocol for organoid derivation, expansion, and cryopreservation. We evaluated the success rate of organoid establishment and the histo-molecular fidelity to the parental tumor. These early results demonstrate promising engraftment efficiency and maintenance of tumor-specific markers across passages. Our work highlights the potential of PDOs as a reliable and scalable platform for translational research in HCC, setting the stage for future applications in drug screening and biomarker discovery.

Indexed as

Carcinoma, HepatocellularLiver NeoplasmsOrganoidsAgedAnimalsFemaleHumansMaleMiddle Agedcirrhosishepatocellular carcinomaliver tumororganoidstranslational research

Identifiers

PMID41597200
PMCPMC12839850

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.