Evidence map›Paper›PMID 41600575›Full record

ReviewToxics2025

Glyphosate as an Emerging Environmental Pollutant and Its Effects on Breast Cancer Cell Proliferation: A Systematic Literature Review of Preclinical Evidence.

Mario A Alcalá-Pérez, Gustavo A Hernández-Fuentes, Idalia Garza-Veloz, Uriel Diaz-Llerenas, Margarita L Martinez-Fierro, José Guzmán-Esquivel, Fabian Rojas-Larios, Ángel A Ramos-Organillo, Kayim Pineda-Urbina, José M Flores-Álvarez and 4 more

Abstract readReview
In one paragraph

Review in Toxics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. RoundupFrontiers in endocrinology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Mario A Alcalá-PérezMolecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Autonomous University of Zacatecas, Zacatecas 98160, Mexico.ORCID 0009-0004-4505-7892
Gustavo A Hernández-FuentesDepartment of Molecular Medicine, School of Medicine, University of Colima, Colima 28040, Mexico.ORCID 0000-0003-4685-3095
Idalia Garza-VelozMolecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Autonomous University of Zacatecas, Zacatecas 98160, Mexico.ORCID 0000-0002-6307-1696
Uriel Diaz-LlerenasMolecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Autonomous University of Zacatecas, Zacatecas 98160, Mexico.ORCID 0009-0007-4374-4578
Margarita L Martinez-FierroMolecular Medicine Laboratory, Academic Unit of Human Medicine and Health Sciences, Autonomous University of Zacatecas, Zacatecas 98160, Mexico.ORCID 0000-0003-1478-9068
José Guzmán-EsquivelClinical Epidemiology Research Unit, Mexican Institute of Social Security, Villa de Alvarez 28984, Colima, Mexico.ORCID 0000-0002-6727-0051
Fabian Rojas-LariosDepartment of Molecular Medicine, School of Medicine, University of Colima, Colima 28040, Mexico.ORCID 0000-0003-1744-9173
Ángel A Ramos-OrganilloFaculty of Chemical Sciences, University of Colima, Colima 28400, Mexico.ORCID 0000-0002-9690-608X
Kayim Pineda-UrbinaFaculty of Chemical Sciences, University of Colima, Colima 28400, Mexico.ORCID 0000-0003-3462-6020
José M Flores-ÁlvarezFaculty of Chemical Sciences, University of Colima, Colima 28400, Mexico.ORCID 0000-0002-7117-6498
Juan P Mojica-SánchezDepartment of Engineering in Sustainable Agricultural Innovation, Tecnológico Nacional de México/Instituto Tecnológico José Mario Molina Pasquel y Henríquez, Unidad Académica Tamazula, Carretera Tamazula-Santa Rosa No. 329, Tamazula de Gordiano 49650, Jalisco, Mexico.
Jorge A Cárdenas-MagañaDepartment of Electromechanical Engineering, Tecnológico Nacional de México/Instituto Tecnológico José Mario Molina Pasquel y Henríquez, Unidad Académica Tamazula, Carretera Tamazula-Santa Rosa No. 329, Tamazula de Gordiano 49650, Jalisco, Mexico.ORCID 0000-0001-9114-8550
Cesar A Villa-MartínezFaculty of Chemical Sciences, University of Colima, Colima 28400, Mexico.ORCID 0009-0003-2414-835X
Iván Delgado-EncisoDepartment of Molecular Medicine, School of Medicine, University of Colima, Colima 28040, Mexico.ORCID 0000-0001-9848-862X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The widespread use of glyphosate-based herbicides (GBHs) has raised concerns about their potential role in hormone-sensitive cancers such as breast cancer. This systematic review aimed to evaluate preclinical evidence on the effects of glyphosate (pure compound) or glyphosate-based herbicide formulations (GBHs) exposure on breast cancer cell proliferation and related molecular pathways. A structured search was conducted across PubMed, ScienceDirect, and Springer Nature Link, Web of Science databases, covering studies published up to 9 November 2025, following a PROSPERO-registered protocol (ID: CRD42021238350). Eligible studies included original in vitro and in vivo preclinical research using human breast cancer cell lines (e.g., MCF-7, T47D, MDA-MB-231, MCF-12A, and MCF-10A) or relevant animal models. Outcomes assessed included cell viability, proliferation, tumor growth, apoptosis, cell cycle regulation, and molecular markers associated with endocrine signaling. Two reviewers independently screened and extracted data, resolving disagreements via discussion or third-party adjudication. From an initial pool of 699 articles, seven in vitro studies met the inclusion and quality criteria. Glyphosate exposure demonstrated weak estrogenic activity in ER-positive breast cancer cells, primarily via ERα modulation and altered gene expression related to proliferation and DNA repair. GBHs showed greater cytotoxic and epigenetic effects in non-tumorigenic cells, often independent of ER signaling. No included study employed in vivo breast cancer models. Overall, preclinical evidence suggests glyphosate may act as a weak endocrine disruptor under specific conditions, but findings are limited by the short-term in vitro designs, heterogeneous methodologies, and lack of chronic or in vivo data. Further research using long-term exposure and animal models is needed to clarify potential risks and inform regulatory and public health decisions.

Indexed as

breast cancerbreast cancer cellscancer cell proliferationendocrine disruptorsglyphosateglyphosate-based herbicidestumor growth

Identifiers

PMID41600575
PMCPMC12846237

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.