ArticleJournal of clinical laboratory analysis2026
Validation of Hexaplex Recombinase Polymerase Amplification Assay for the Detection of Carbapenemase Genes in Klebsiella pneumoniae Isolates From Pediatric Inpatients.
Article in Journal of clinical laboratory analysis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- Duplex Recombinase Polymerase Amplification-Lateral Flow Assay for Rapid Visual Detection of Critical Antibiotic Resistance Genes in Escherichia coli and Klebsiella pneumoniae From Positive Blood Cultures.Journal of clinical laboratory analysis · 2026Article
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Authors and funding
2 authors.
Funding
Abstract
backgroundThe carbapenemase-producing Klebsiella pneumoniae (CRKP) is a global health problem associated with nosocomial and opportunistic infection and gastrointestinal colonization. A rapid detection method for carbapenemase genes is necessary for effective treatment and to prevent dissemination of carbapenemase genes.
methodsIn this study, a hexaplex recombinase polymerase amplification assay was established for detection of five common carbapenemase genes (bla
resultsThe duplex to hexaplex primer concentrations were successfully optimized. Single-tube RPA reaction could be completed at 37°C in 25-30 min. The limit of detection of each target varied from 0.1 to 10 ng. No cross-reactivity of hexaplex primers was observed against five Gram-positive and four Gram-negative pathogenic bacteria in pediatric infections. The sensitivity and specificity were 100% with perfect concordance with both reference methods.
conclusionTherefore, the hexaplex RPA has potential for rapid carbapenemase genes detection in limited resource settings.
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