Evidence map›Paper›PMID 41612039›Full record

ArticleCommunications biology2026

PTBP1 supports mouse spermatogenesis by facilitating cytoskeletal organization through the mTORC2-PKCα pathway in Sertoli cells.

Manabu Ozawa, Jumpei Taguchi, Hideto Mori, Mio Kikuchi, Masafumi Muratani, Takuya Yamamoto, Yasuhiro Yamada, Nobuaki Yoshida, Masahito Ikawa

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Manabu OzawaCore Laboratory for Developing Advanced Animal Models, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan. semil@ims.u-tokyo.ac.jp.ORCID http://orcid.org/0000-0003-3284-7161
Jumpei TaguchiCore Laboratory for Developing Advanced Animal Models, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.ORCID http://orcid.org/0009-0007-3115-6541
Hideto MoriPremium Research Institute for Human Metaverse Medicine (WPI-PRIMe), The University of Osaka, Osaka, Japan.ORCID http://orcid.org/0000-0003-4849-0056
Mio KikuchiCore Laboratory for Developing Advanced Animal Models, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
Masafumi MurataniDepartment of Genome Biology, Transborder Medical Research Center, Institute of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan.ORCID http://orcid.org/0000-0002-0276-8000
Takuya YamamotoDepartment of Life Science Frontiers, Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, Japan.ORCID http://orcid.org/0000-0002-0022-3947
Yasuhiro YamadaDepartment of Molecular Pathology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.ORCID http://orcid.org/0000-0002-2463-1478
Nobuaki YoshidaLaboratory of Developmental Genetics, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.
Masahito IkawaLaboratory of Reproductive Systems, Center for Experimental Medicine and Systems Biology, Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.ORCID http://orcid.org/0000-0001-9859-6217

Funding

Functional analysis of novel testis-expressed secreted and transmembrane proteinsR01HD088412 · NICHD · BAYLOR COLLEGE OF MEDICINE · PI MARTIN M. MATZUK · 2016 to 2026
$4.5M
Gates Foundation INV-001902MEXT | Japan Society for the Promotion of Science (JSPS) 20H03162MEXT | Japan Society for the Promotion of Science (JSPS) 23H02391MEXT | Japan Society for the Promotion of Science (JSPS) JP21H05033MEXT | JST | Core Research for Evolutional Science and Technology (CREST) JPMJCR21N1U.S. Department of Health & Human Services | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) R01HD088412
6 · The paper itself

Abstract

Somatic Sertoli cells in the seminiferous tubule provide structural and paracrine support for spermatogenesis. Proper regulation of Sertoli cell cytoskeleton dynamics is essential for this function; however, the regulatory mechanisms remain unclear. Here, we found that polypyrimidine tract-binding protein 1 (PTBP1) maintained cytoskeletal integrity in Sertoli cells via mTORC2 signaling. Sertoli cell-specific Ptbp1 deletion in mice resulted in germ cell loss, blood-testis barrier disruption, and male infertility. Transcriptomic analysis of purified Sertoli cells revealed the dysregulation of cytoskeletal and adhesion-related genes. RNA immunoprecipitation sequencing demonstrated that PTBP1 bound to Rictor mRNA. PTBP1 loss reduced RICTOR protein expression and downstream PKCα activation, impairing the F-actin cytoskeleton. Cytoskeletal defects caused by PTBP1 deficiency were restored by constitutively active PKCα, confirming the functional relevance of the mTORC2-PKCα axis. These findings highlighted PTBP1's role as a post-transcriptional regulator of male fertility through the maintenance of Sertoli cell architecture and spermatogenesis.

Indexed as

CytoskeletonHeterogeneous-Nuclear RibonucleoproteinsMechanistic Target of Rapamycin Complex 2Polypyrimidine Tract-Binding ProteinProtein Kinase C-alphaSertoli CellsSpermatogenesisAnimalsBlood-Testis BarrierMaleMiceMice, KnockoutRapamycin-Insensitive Companion of mTOR ProteinSignal TransductionHeterogeneous-Nuclear RibonucleoproteinsMechanistic Target of Rapamycin Complex 2Polypyrimidine Tract-Binding ProteinProtein Kinase C-alphaPtbp1 protein, mouseRapamycin-Insensitive Companion of mTOR Proteinrictor protein, mouse

Identifiers

PMID41612039
PMCPMC12957323

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.