Evidence map›Paper›PMID 41613445›Full record

ArticleFundamental research2025

Detection of antibiotic-resistance genes in bacterial pathogens using a Cas12a/3D DNAzyme colorimetric paper sensor.

Hua Gao, Yanan Li, Yaqiong Li, Ke Qu, Kaixiang Zhang, Jinghong Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fundamental research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Hua GaoSchool of Pharmaceutical Sciences, Key laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Zhengzhou University, Zhengzhou 450001, China.
Yanan LiSchool of Pharmaceutical Sciences, Key laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Zhengzhou University, Zhengzhou 450001, China.
Yaqiong LiSchool of Pharmaceutical Sciences, Key laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Zhengzhou University, Zhengzhou 450001, China.
Ke QuCollege of Materials and Chemistry & Chemical Engineering, Chengdu University of Technology, Chengdu 610059, China.
Kaixiang ZhangSchool of Pharmaceutical Sciences, Key laboratory of Targeting Therapy and Diagnosis for Critical Diseases, Zhengzhou University, Zhengzhou 450001, China.
Jinghong LiDepartment of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing 100084, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The rapid detection of antibiotic-resistant genes in bacterial pathogens is critical in combating global health crises. Herein, we report a CRISPR/Cas12a-based colorimetric paper sensor, where the trans-cleavage activity of Cas12a was post-amplified by rolling circle replication, resulting in the generation of a 3D DNAzyme. The 3D DNAzyme adhered strongly to the paper surface, creating a highly bioactive paper sensor containing high densities of functional DNAzymes. This assay was effective for the rapid detection of the antibiotic-resistant gene,

Indexed as

3D DNAzymeAntibiotic susceptibility testCRISPR-Cas12aPaper sensorRolling circle amplification

Identifiers

PMID41613445
PMCPMC12848165

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.