Evidence map›Paper›PMID 41618132›Full record

ArticleBMC microbiology2026

Metabolomic profiling of Bacillus amyloliquefaciens MU-10 and its antagonistic effects on Cercospora arachidicola.

Taswar Ahsan, Bingbing Liang, Xiaozhou Liu, Chaoqun Zang, Yuqian Huang, Chen Wang, Ao Ding, Chunhao Liang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Taswar Ahsan *Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, P. R. China.
Bingbing Liang *Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, P. R. China.
Xiaozhou LiuInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, P. R. China.
Chaoqun ZangInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, P. R. China.
Yuqian HuangCollege of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.
Chen WangCollege of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.
Ao DingCollege of Plant Protection, Shenyang Agricultural University, Shenyang, 110866, China.
Chunhao LiangInstitute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang, 110161, P. R. China. liangchunhao99@126.com.

Funding

Basic Research Expenses of Liaoning Academy of Agricultural Sciences 2021GQ1903Major Agricultural Projects of Liaoning Province 2023JH1/10200002Science and Technology Project of Liaoning Province 2023JH1 / 10200002
6 · The paper itself

Abstract

backgroundEarly leaf spot disease in peanut severely impacts crop yield and quality. In response, biological control by Bacillus strains offers an alternative approach to replace harmful chemical fungicides, as these bacteria produce bioactive secondary metabolites. The effectiveness of these metabolites greatly relies on the submerged fermentation process, which in turn depends on optimizing the nutrient media-especially since there is a significant difference between nutrient-optimized fermentation medium (OP) and nonoptimized fermentation medium (NOP).

resultsIn the present study, we isolated an antagonistic strain, Bacillus amyloliquefaciens MU-10, from soil and identified it. According to in vitro experiments, strain MU-10 exhibited potent antifungal activity against C. arachidicola, with an inhibition zone of 76 mm. Similarly, under greenhouse conditions, the disease control efficiency of the MU-10 strain against peanut leaf spot disease was 93.33%. SEM analysis revealed that the hyphae of the pathogen were destroyed and that the effect of MU-10 reduced the abundance of spores. UHPLC-MS/MS was used to elucidate the bioactive secondary metabolites produced by strain MU-10 in OP and NOP submerged fermentation. A total of 831 metabolites were detected in OP, 32% of which were classified as organic acids. Among these were 83 differentially expressed metabolites (DEMs). According to the VIP scores, 12 metabolites were identified as key contributors to group separation, including the upregulated 3-O-acetylisopimaric acid and vanillylamine. Conversely, pyrrole-2-carboxylic acid was identified as a downregulated VIP metabolite in OP. The receiver operating characteristic (ROC) curves confirmed all the metabolic results. Pure compound validation was confirmed by the use of commercially synthesized vanillylamine, pyrrole-2-carboxylic acid, and syringic acid as key antifungal agents.

conclusionNutrient-optimized fermentation significantly increased the production of Bacillus-based biocontrol products. This improvement represents a significant advancement in sustainable, environmentally friendly crop disease management, providing an effective solution for controlling early leaf spot in peanuts.

Indexed as

AntibiosisBacillus amyloliquefaciensCercosporaMetabolomicsPlant DiseasesAntifungal AgentsArachisCulture MediaFermentationMetabolomeSecondary MetabolismSoil MicrobiologyTandem Mass SpectrometryAntifungal AgentsCulture MediaATP-binding cassetteBiocontrolCercospora arachidicolaPeanut early leaf spotPyrrole-2-carboxylic acidUHPLC/MS-MSVanillylamine

Identifiers

PMID41618132
PMCPMC12973580

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.