Evidence map›Paper›PMID 41627767›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Enzyme Assays for Phosphoinositide Kinases and Phosphatases and Inhibitor Screens.

Mengjin Gao, Manoj Koirala, Natalie B Warren, Jacqueline C Bede, Igor Cestari

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Mengjin GaoInstitute of Parasitology, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Manoj KoiralaInstitute of Parasitology, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Natalie B WarrenDepartment of Plant Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Jacqueline C BedeDepartment of Plant Science, McGill University, Sainte-Anne-de-Bellevue, QC, Canada.
Igor CestariInstitute of Parasitology, McGill University, Sainte-Anne-de-Bellevue, QC, Canada. igor.cestari@mcgill.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In vitro enzyme assays provide sensitive and quantitative approaches to studying activity, identifying substrates or performing inhibitor analysis. These approaches can help to study enzymes that regulate different cellular processes in an organism. In addition, enzyme assays can be used to screen protein inhibitors to identify novel drug candidates. In eukaryotes, phosphatidylinositol kinase and phosphatase are enzymes that regulate the level of phosphorylated phosphatidylinositol, which controls several cellular processes. They also regulate several processes in many pathogens, such as kinetoplastid and apicomplexan parasites, and are validated drug targets. Here, we describe protocols to determine the activity of purified phosphoinositide kinases and phosphatases and screens to identify potential inhibitors. The assays are luminescence- and absorbance-based to measure the activity of phosphatidylinositol kinase and phosphatase enzymes, respectively. These assays can be adapted to other protein kinases and phosphatases.

Indexed as

Enzyme AssaysEnzyme InhibitorsPhosphoric Monoester HydrolasesAnimalsKineticsPhosphatidylinositolsPhosphorylationEnzyme InhibitorsPhosphatidylinositolsPhosphoric Monoester HydrolasesEnzyme assayEnzyme inhibitorInositol phosphatesMichaelis-Menten kineticsPhosphatidylinositol

Identifiers

PMID41627767

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.