Evidence map›Paper›PMID 41629985›Full record

ArticleBMC pharmacology & toxicology2026

Elucidating the mechanisms by which acetyl tributyl citrate affects fracture healing: a comprehensive network toxicology study.

Yining Chen, Caiyun Huang, Yuan Zhou, Zhongyuan Liu, Xialin Tang, Yanqiu Li, Chenkang Lu, Qiwang He

Abstract read
In one paragraph

Article in BMC pharmacology & toxicology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yining Chen *Hubei University of Chinese Medicine, Wuhan, 430061, China.
Caiyun Huang *Department of Blood Transfusion, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, 441021, China.
Yuan ZhouHubei University of Chinese Medicine, Wuhan, 430061, China.
Zhongyuan LiuCapital Medical University, Beijing, 100069, China.
Xialin TangHubei Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Hubei University of Chinese Medicine, Hubei Key Laboratory of Theory and Application Research of Liver and Kidney in Traditional Chinese Medicine, Hubei Province Academy of Traditional Chinese Medicine, Wuhan, 430061, China.
Yanqiu LiHubei University of Chinese Medicine, Wuhan, 430061, China.
Chenkang LuHubei University of Chinese Medicine, Wuhan, 430061, China.
Qiwang HeHubei University of Chinese Medicine, Wuhan, 430061, China. 18257206675@163.com.

Funding

Administration of Traditional Chinese Medicine of Hubei Province for Youths ZY2023Q001Natural Science Foundation of Hubei Province for Youths 2022CFB923
6 · The paper itself

Abstract

backgroundIn this study, we explored the potential risk effects of acetyl tributyl citrate (ATBC) on fracture healing through the method of network toxicology.

methodsATBC-related targets were retrieved from the ChEMBL, Swiss Target Prediction, and STITCH databases, whereas fracture healing-related targets were obtained from the GeneCards, OMIM, and GEO databases. Core targets were identified through protein-protein interaction network construction using the STRING database and visualized using the Cytoscape software, followed by GO and KEGG enrichment analyses. Additionally, in-depth analyses, including immune infiltration profiling, gene set enrichment analysis, and ceRNA regulatory network construction, were conducted. Finally, molecular docking and dynamics simulations were performed to determine the binding efficiency and stability for the binding between ATBC and its core targets.

resultsA total of 50 overlapping targets were identified, from which six core targets were selected. Using differential expression analysis, five core targets (HDAC2, HDAC3, KAT2B, SMARCA4, and TP53) were further refined. Enrichment analysis suggested potential mechanisms related to the thyroid hormone signaling pathway, the Notch signaling pathway, and the cell cycle. Molecular docking and dynamics simulations confirmed certain binding interactions between ATBC and the core targets.

conclusionsWe provided an integrated network toxicology analysis framework coupled with a molecular dynamics evaluation framework to elucidate the underlying molecular mechanisms through which ATBC interferes with fracture healing, thereby providing novel therapeutic strategies for clinical intervention. However, the findings require further experimental validation and clinical confirmation to establish translational relevance.

trial registrationNot applicable.

Indexed as

Fracture HealingTrialkyltin CompoundsAnimalsHumansMolecular Docking SimulationMolecular Dynamics SimulationProtein Interaction MapsTrialkyltin CompoundsATBCFracture healingMolecular dynamics simulationNetwork toxicology

Identifiers

PMID41629985
PMCPMC12955142

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.