ArticleAMB Express2026
Rumen-protected betaine supplementation improves ileal function by affecting microbe and metabolite in Tibetan sheep.
Article in AMB Express, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
The research explored the effect of dietary rumen-protected betaine (RPB) supplementation on development and function of ileum in Tibetan sheep. Sixty male lambs (17.72 ± 0.19 kg, aged 2 months) were randomly divided into control group (Ctrl) and RPB group (0.08% Bet). The adaptation feeding period was 10 days, and the experimental period was 90 days. Results suggested that dietary RPB significantly increased villus height, villus height-to-crypt depth (VH/CD) ratio, total antioxidant capacity (T-AOC) (P < 0.05), and decreased malondialdehyde (MDA), lipopolysaccharide (LPS), tumor necrosis factor-α (TNF-α) (P < 0.05), as well as altered the composition of short-chain fatty acids (SCFAs). Importantly, expression of ileal barrier-related gene (Claudin-1) was elevated (P < 0.05). Microbial analyses showed RPB supplementation increased the relative abundances of Aeriscardovia and Bifidobacterium. Several differential metabolites, including Cer 18:1;2O/18:0, Cer 18:1;2O/24:2 and (±)13-HpODE, were significantly increased in the RPB group (P < 0.05). The VH/CD was positively correlated with Bifidobacterium, Cer 18:1;2O/18:0, Cer 18:1;2O/24:2 and (±)13-HpODE (r > 0.5). To sum up, our results suggest that the dietary RPB supplementation could affect the ileal SCFA concentration by modulating the microbial community and regulating metabolism, thereby contributing to ileal development and barrier function. This study offers new evidence supporting the use of RPB as a potential application for Tibetan sheep.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.