Evidence map›Paper›PMID 41644937›Full record

ReviewStem cell reviews and reports2026

The Impact of Large-Scale Expansion on the Functional Properties of Mesenchymal Stem Cells.

Guadalupe Antonio-Ríos, Rosa María Ribas-Aparicio, Gerardo Leyva-Gómez, Gloria Soldevila, Karla Alejandra Espinoza Dueñas, Cynthia Georgina Trejo-Iriarte, Maykel González-Torres

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Stem cell reviews and reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Guadalupe Antonio-RíosLaboratorios de Producción y Control de Biológico, Biotecnología Molecular y Farmacéutica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, 11340, México.
Rosa María Ribas-AparicioLaboratorios de Producción y Control de Biológico, Biotecnología Molecular y Farmacéutica, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, 11340, México.
Gerardo Leyva-GómezDepartamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, México.
Gloria SoldevilaDepartamento de Inmunología y Laboratorio Nacional de Citometría de Flujo, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Circuito Escolar s/n, Ciudad Universitaria, Colonia Copilco, Delegación Coyoacán, Ciudad de México, 04510, Mexico.
Karla Alejandra Espinoza DueñasCentro de Graduados e Investigación, Instituto Tecnológico de Tijuana, Blvd. Limón Padilla S/N, Mesa de Otay, Apartado Postal 1166, Tijuana, BC, 22000, México.
Cynthia Georgina Trejo-IriartePrograma de Cirugía Dental, Laboratorio de Investigación en Odontología Almaraz, Facultad de Estudios Superiores Iztacala, UNAM, Cuautitlán Izcalli, Estado de México, 54714, México.
Maykel González-TorresDepartamento de Ciencias Químicas, Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Estado de México, México. maykel.gonzalez@unam.mx.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stem cells (MSCs) are highly valuable for their potential in cell therapy and tissue engineering because of their self-renewal, multilineage differentiation, and immunomodulatory capabilities. Adipose-derived mesenchymal stem cells (AD-MSCs) are advantageous in regenerative medicine because of their accessibility and ease of isolation. However, the clinical application of MSCs faces challenges related to large-scale culture (LSC) expansion, which is required to generate enough cells for transplantation but also decreases their therapeutic properties. This review assesses the impact of LSC on MSC functionality, differentiation potential, and immunomodulatory properties, and identifies key factors, such as metabolic shifts, genetic instability, and altered secretory profiles, that can compromise their therapeutic potential. We explored how prolonged in vitro passaging decreases MSC functionality and increases the risk of genetic alterations. In addition, strategies to preserve the efficacy of MSCs during scaling are discussed. A comprehensive literature review was conducted using PubMed, focusing on in vitro and in vivo studies that evaluated the effects of LSC on MSCs. These findings provide insights into optimizing culture protocols to maintain the clinical efficacy of AD-MSCs in regenerative therapies, addressing the critical need to balance large-scale expansion and functional integrity.

Indexed as

Cell Culture TechniquesMesenchymal Stem CellsRegenerative MedicineCell Self RenewalHumansImmune ToleranceImmunosuppression TherapyAdipose-derived stem cellsCell functionalityLarge-scale culture expansionMesenchymal stem cellsRegenerative medicineTherapeutic potential

Identifiers

PMID41644937
PMCPMC12960335

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.