Evidence mapPaperPMID 41651960Full record

ArticleScientific reports2026

Bee pollen-derived peptide with dual DPP-IV Inhibition and glucose transport modulation.

Usa Mongkolnkrajang, Kittisak Kuptawach, Papassara Sangtanoo, Tanatorn Saisavoey, Patamalai Boonserm, Onrapak Reamtong, Piroonporn Srimongkol, Aphichart Karnchanatat

Abstract read
In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Usa MongkolnkrajangProgram in Biotechnology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.
Kittisak KuptawachProgram in Biotechnology, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.
Papassara SangtanooCenter of Excellence in Bioconversion and Bioseparation for Platform Chemical Production, The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.
Tanatorn SaisavoeyCenter of Excellence in Bioconversion and Bioseparation for Platform Chemical Production, The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.
Patamalai BoonsermCenter of Excellence in Bioconversion and Bioseparation for Platform Chemical Production, The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.
Onrapak ReamtongDepartment of Molecular Tropical Medicine and Genetics, Faculty of Tropical Medicine, Mahidol University, 420/6 Ratchawithi Road, Ratchathewi, Bangkok, 10400, Thailand.
Piroonporn SrimongkolHigh-Value Food from Mushrooms and Bioactive Plants in the Green Economy Value Chain Research Group, The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand.
Aphichart KarnchanatatCenter of Excellence in Bioconversion and Bioseparation for Platform Chemical Production, The Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, 254 Phayathai Road, Pathumwan, Bangkok, 10330, Thailand. Aphichart.K@chula.ac.th.

Funding

The 90th Anniversary of Chulalongkorn University Fund, Thailand (Ratchadaphiseksomphot Endowment Fund) GCUGR1125642023MThe Thailand Science Research and Innovation Fund Chulalongkorn University BCG_FF_69_038_6100_002
6 · The paper itself

Abstract

This study investigates the potential of bee pollen protein hydrolysate (BPPH) as a natural source of bioactive peptides capable of inhibiting dipeptidyl peptidase IV (DPP-IV) for the management of type 2 diabetes mellitus (T2DM), a metabolic disorder characterized by insulin resistance and hyperglycemia. BPPH was generated through pepsin–pancreatin digestion, followed by ultrafiltration and RP-HPLC purification. LC-Q-TOF-MS/MS analysis identified Ala-Thr-His-Ala-Leu-Leu-Ala (ATHALLA, AA-7) as a predominant peptide associated with DPP-IV inhibitory activity. AA-7 exhibited strong DPP-IV inhibitory activity (IC50 = 52.63 ± 2.32 µM) relative to the reference inhibitor diprotin A (IC50 = 22.4 ± 1.29 µM). Molecular docking predicted stable binding of AA-7 within the DPP-IV catalytic pocket, mediated by hydrogen bonding and hydrophobic interactions with key residues. AA-7 also modulated glucose uptake in Caco-2 cells, influencing SGLT1 and GLUT2 gene expression in a dose-dependent manner. Docking analysis suggested potential interactions with selected SGLT1 and GLUT2 residues, providing structural support for the observed cellular responses rather than definitive mechanistic evidence. In silico ADMET analysis indicated poor passive membrane permeability and limited predicted intestinal absorption, along with minimal CYP450 interactions and low predicted toxicity, highlighting potential pharmacokinetic limitations while supporting a favorable safety profile. These findings highlight AA-7 as a dual-action peptide with demonstrated DPP-IV inhibitory activity and the ability to modulate glucose transport in vitro, supporting the potential of bee-pollen-derived peptides for glycemic regulation and functional food or nutraceutical applications.

Indexed as

Dipeptidyl Peptidase 4Dipeptidyl-Peptidase IV InhibitorsGlucosePeptidesPollenAnimalsBeesBioactive Peptides, DietaryBiological TransportCaco-2 CellsHumansMolecular Docking SimulationSodium-Glucose Transporter 1Bioactive Peptides, DietaryDipeptidyl Peptidase 4Dipeptidyl-Peptidase IV InhibitorsGlucosePeptidesSodium-Glucose Transporter 1Bee pollenBioactive peptideDPP-IV inhibitorGlucose uptakeGLUT2SGLT1Type 2 diabetes mellitus

Identifiers

PMID41651960
PMCPMC12936078

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.