Evidence mapPaperPMID 41660412Full record

ArticleFrontiers in nutrition2026

The effects of time-restricted feeding on early phases of carcinogenesis in rat liver and colon.

Nadia Malakmahmoudi, Roberta Pisu, Andrea Barbarossa, Francesca Pisu, Giuseppe S Porcu, Ezio Laconi, Andrea Perra, Sergio Uzzau, Fabio Marongiu

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Article in Frontiers in nutrition, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Nadia MalakmahmoudiDepartment of Biomedical Sciences, University of Cagliari, Cagliari, Italy.
Roberta PisuDepartment of Biomedical Sciences, University of Cagliari, Cagliari, Italy.
Andrea BarbarossaDepartment of Biomedical Sciences, University of Cagliari, Cagliari, Italy.
Francesca PisuDepartment of Histopathology, "A. Businco" Oncology Hospital, Azienda Ospedaliera Brotzu, Cagliari, Italy.
Giuseppe S PorcuDepartment of Histopathology, "A. Businco" Oncology Hospital, Azienda Ospedaliera Brotzu, Cagliari, Italy.
Ezio LaconiDepartment of Biomedical Sciences, University of Cagliari, Cagliari, Italy.
Andrea PerraDepartment of Biomedical Sciences, University of Cagliari, Cagliari, Italy.
Sergio UzzauDepartment of Biomedical Sciences, University of Sassari, Sassari, Italy.
Fabio MarongiuDepartment of Biomedical Sciences, University of Cagliari, Cagliari, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and aims: High-fat diets are established contributors to carcinogenesis through mechanisms involving altered lipid metabolism, metabolic stress, and chronic inflammation. Time-restricted feeding (TRF) has emerged as a promising non-pharmacological strategy to improve metabolic health and potentially mitigate cancer risk by optimizing glycemic control, lipid profile, and inflammatory triggers. Despite these potential benefits, the direct impact of TRF on early-stage carcinogenesis, particularly in the context of obesogenic dietary conditions, remains inadequately explored. This study aimed to investigate the effects of TRF on the development of preneoplastic lesions in the liver and colon in male and female rats exposed to high-fat (HFD) and low-fat (LFD) diets. Methods: Carcinogenesis was initiated using diethyl nitrosamine (DENA) for the liver and azoxymethane (AOM) for the colon. Rats were assigned to ad libitum feeding (AdL) or TRF (8-h feeding window) following carcinogen exposure and were euthanized at 6 or 9 months for assessment. Pre-neoplastic lesions in the liver were evaluated by glutathione S-transferase placental form (GSTP)-positive staining, while aberrant crypt foci (ACF) were analyzed in the colon. Additionally, hepatic steatosis and metabolic parameters were assessed to determine the impact of TRF. Results: No differences were observed in the incidence, size, or distribution of GSTP-positive lesions in the liver or ACF in the colon between TRF and AdL groups. TRF also failed to reduce hepatic steatosis or improve serum lipid profiles in animals fed an HFD. Conclusion: Our findings indicate that TRF does not significantly alter the development of early preneoplastic lesions in the liver or colon, regardless of dietary fat content, and further suggest that this dietary regimen is insufficient alone to counteract metabolic dysfunctions induced by highly obesogenic diets in rats.

Indexed as

colonearly carcinogenesishigh-fat dietlivertime-restricted feeding

Identifiers

PMID41660412
PMCPMC12875941

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.