ArticleFrontiers in nutrition2026
Fractional ethanol precipitation modulates the structure and function of
Article in Frontiers in nutrition, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Microwave-vacuum drying modulates the supramolecular organization and biofunctionality of β-glucans from edible mushrooms.Food chemistry: X · 2026Article
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4 authors.
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Abstract
Introduction: This study aimed to systematically fractionate Methods: Polysaccharides (CCPJ-40, -60, -80) were obtained with 40%, 60%, and 80% ethanol. Their chemical properties (yield, monosaccharide composition, molecular weight) and in vitro bioactivities were assessed. The optimal fraction was investigated in a dextran sulfate sodium (DSS)-induced Caco-2 cell model. Results: The results revealed that the ethanol concentration significantly affected the polysaccharide yield, chemical composition, and bioactivities. Notably, CCPJ-80 exhibited the highest yield and total sugar content. Monosaccharide composition analysis indicated that both CCPJ-40 and CCPJ-80 were composed of L-fucose, L-arabinose, galactose, glucose, xylose, mannose, ribose, and D-glucuronic acid, whereas CCPJ-60 additionally contained rhamnose. Regarding molecular weight distribution, CCPJ-40 had a high molecular weight and was relatively polydisperse, while CCPJ-60 and CCPJ-80 had lower molecular weights with good homogeneity. In the Discussion: This study elucidates the regulatory effect of ethanol precipitation concentration on the physicochemical properties and functional activities of CC mycelial polysaccharides, and reveals the potential of CCPJ-80 to ameliorate ulcerative colitis by modulating oxidative stress and mitochondrial function pathways, providing a scientific basis for developing CCPJ as a functional food or therapeutic agent.
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