Evidence map›Paper›PMID 41660728›Full record

ArticleJournal of medical virology2026

Development of a Fully Automated, High-Throughput Molecular Assay for Detection of Rat Hepatitis E Virus in Routine Diagnostics.

Jessica Panajotov, Katja Giersch, Lisa Sophie Pflüger, Dominik Nörz, Moritz Grunwald, Hui Ting Tang, Marco Kaiser, Sven Pischke, Rainer G Ulrich, Susanne Pfefferle and 5 more

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of medical virology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jessica PanajotovGerman Federal Institute for Risk Assessment (BfR), Berlin, Germany.
Katja GierschInstitute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.ORCID https://orcid.org/0000-0003-3989-0170
Lisa Sophie PflügerInstitute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.
Dominik NörzInstitute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.
Moritz GrunwaldInstitute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.
Hui Ting TangInstitute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.
Marco KaiserTIB-Molbiol Syntheselabor GmbH, Berlin.
Sven PischkeDepartment of Medicine, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.
Rainer G UlrichGerman Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems site, Germany.
Susanne PfefferleInstitute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.
Julian Schulze Zur WischDepartment of Medicine, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.
Victor Max CormanInstitute of Virology, Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany.
Martin AepfelbacherInstitute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.
Reimar JohneGerman Federal Institute for Risk Assessment (BfR), Berlin, Germany.
Marc LütgehetmannInstitute of Medical Microbiology, Virology and Hygiene, University Medical Center Hamburg-Eppendorf (UKE), Hamburg, Germany.

Funding

DFG iDfellows493624519DFG SFB1648/12024-512741711DZIF TTU05.833;TTU05.834German Federal Ministry of Education and Research 01KI2103NUM-SAR 01KX2521
6 · The paper itself

Abstract

Recently, cases of human infection with rat hepatitis E virus (ratHEV, Rocahepevirus ratti) have been reported worldwide. Due to the significant genetic differences between ratHEV and human HEV genotypes 1-4 (Paslahepevirus balayani), current HEV diagnostic assays are unable to detect ratHEV. The aim was to establish and validate a laboratory-developed ratHEV RT-qPCR assay for use with human plasma and stool samples on a fully automated, high-throughput platform. Published primers and probes were optimized for use on cobas 5800/6800/8800 systems using European Union In Vitro Diagnostics Regulation (IVDR)-grade reagents, including an RNA full-process inhibition control. Analytical sensitivity (21 repeats), linear range (five repeats) and precision (three repeats over 3 days) were evaluated using viral particles from cell culture (MN450851.1). The inclusivity was verified using DNA oligonucleotides and known positive samples (rat liver and human serum). The limits of detection were 98.9 copies/ml in plasma and 60.3 copies/ml in stool, and the assay showed excellent linearity over at least 5 log (r

Indexed as

Automation, LaboratoryHepatitis EHepatitis E virusHigh-Throughput Screening AssaysMolecular Diagnostic TechniquesReal-Time Polymerase Chain ReactionAnimalsDNA PrimersFecesHumansPlasmaRatsReproducibility of ResultsRNA, ViralSensitivity and SpecificityDNA PrimersRNA, ViralIVDRratHEVRocahepevirus rattiRT‐qPCR

Identifiers

PMID41660728
PMCPMC12884570

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.