ArticleMetabolomics : Official journal of the Metabolomic Society2026
RPLC- and HILIC-based non-targeted metabolomics workflow for blood microsamples.
Article in Metabolomics : Official journal of the Metabolomic Society, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Recent advances in the bioanalysis of acylcarnitines: Methodologies, challenges, and clinical perspectives.Pharmaceutical science advances · 2026Review
- Column switching liquid chromatography dual mass spectrometry system for simultaneous untargeted metabolomics and targeted exposomics.Nature communications · 2026Article
- An LC-MS untargeted metabolomic comparison between three blood microsampling devices, whole blood, and plasma.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Review
- Optimization of a targeted metabolomics kit for dried blood spots analysis and longitudinal comparison with serum.Metabolomics : Official journal of the Metabolomic Society · 2026Article
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Authors and funding
2 authors.
Funding
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Abstract
introductionBlood microsampling (BμS) has emerged as an alternative to invasive sampling methods, including blood and plasma sampling. Several studies have shown that BμS are suitable alternatives for analyzing endogenous metabolites and for metabolomics applications. Dried blood spots (DBS) have long been used for clinical applications, particularly for newborn screening. New quantitative BμS have emerged, including volumetric absorptive microsampling (VAMS).
objectivesWe aimed to develop an extraction protocol from BµS for non-targeted metabolomics analysis using a reversed-phase liquid chromatography/mass spectrometry (RPLC-MS) method for the mid- to non-polar metabolome and a hydrophilic interaction chromatography/mass spectrometry (HILIC-MS) method for the polar metabolome, based on existing protocols from the literature. To improve coverage, two new HILIC-MS methods have been developed.
methodsWe used an in-house RPLC-MS method for the analysis of mid- to non-polar metabolites. Two new HILIC-MS/MS methods were developed using 73 chemical reference standards of polar metabolites from various classes. To optimize extraction, five procedures were investigated and compared to identify the most appropriate protocol for extracting metabolites from BµS for non-targeted metabolomics analysis. The final workflow was optimized on both DBS and VAMS. RESULTS AND
conclusionWe developed and optimized a 15-minute HILIC-MS method that included column re-equilibration. Our experiments showed that using a 20% H
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