ArticleJournal of extracellular biology2026
Separation Methods Affect Glycan Patterns in Extracellular Vesicle Preparations From Canine Osteoblasts and Osteosarcoma Cells.
Article in Journal of extracellular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
1 citing paper in PubMed.
- Glycosylation of Extracellular Vesicles: Analytical and Translational Insights into Biomarker Discovery and Regenerative Medicine.International journal of molecular sciences · 2026Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Extracellular vesicles (EVs) are nanosized, membrane-enclosed particles released by cells, facilitating intercellular communication via the transfer of bioactive molecules from producing to recipient cells. EVs are involved in several physiological and pathological processes, including cancer progression. The glycosylation of EVs influences the attachment and uptake by recipient cells, among other processes. In this study, we investigated the glycan profile of EVs from canine osteosarcoma (OS) cells and non-cancerous canine osteoblasts in vitro, using lectin blots, following EV separation with ultracentrifugation (UC), size exclusion chromatography (SEC), and a commercial precipitation kit (TEI). Beyond phenotypic differences, purity and yield, separation methods led to heterogeneous protein glycosylation patterns of EV preparations, with the least reproducibility in TEI preparations. In cellular comparison, SEC revealed contrasting results, most consistently showing higher levels of specific sugars in EVs from canine osteoblasts, including the detection of a unique glycoprotein absent in UC and TEI preparations. Osteosarcoma cells and their EVs exhibited a relative reduction of glycoproteins compared to osteoblasts and their EVs. Additionally, specific sugars were more abundant in EV preparations relative to their corresponding cell lysates. In conclusion, this study highlights the critical influence of separation methods on EV characteristics, leading to differing glycan patterns.
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Registered trials
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