Evidence map›Paper›PMID 41673492›Full record

ArticleCell death and differentiation2026

Tagging of C. elegans apoptosis activator EGL-1 BH3-only reveals CED-9 BCL-2-dependent mitochondrial localization and dynamic control of EGL-1 synthesis and degradation in vivo.

Yanwen Jiang, Kate Je Hodgson, Ioannis Segos, Eric J Lambie, Lumeng Yang, Minjia Pan, Alan Greig, Barbara Conradt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death and differentiation, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yanwen JiangCell and Developmental Biology, The Centre for Cell and Molecular Dynamics, Faculty of Life Sciences, University College London, London, UK.ORCID 0000-0002-9894-6560
Kate Je Hodgson *Cell and Developmental Biology, The Centre for Cell and Molecular Dynamics, Faculty of Life Sciences, University College London, London, UK.
Ioannis Segos *Cell and Developmental Biology, The Centre for Cell and Molecular Dynamics, Faculty of Life Sciences, University College London, London, UK.
Eric J LambieCell and Developmental Biology, The Centre for Cell and Molecular Dynamics, Faculty of Life Sciences, University College London, London, UK.
Lumeng YangCell and Developmental Biology, The Centre for Cell and Molecular Dynamics, Faculty of Life Sciences, University College London, London, UK.
Minjia PanCell and Developmental Biology, The Centre for Cell and Molecular Dynamics, Faculty of Life Sciences, University College London, London, UK.
Alan GreigCell and Developmental Biology, The Centre for Cell and Molecular Dynamics, Faculty of Life Sciences, University College London, London, UK.
Barbara ConradtCell and Developmental Biology, The Centre for Cell and Molecular Dynamics, Faculty of Life Sciences, University College London, London, UK. b.conradt@ucl.ac.uk.ORCID 0000-0002-3731-4308

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Ann E. Rougvie · 2012 to 2026
$7.5M
China Scholarship Council (CSC) PhD stipendDeutsche Forschungsgemeinschaft (German Research Foundation) C0204/10-1NIH HHS P40 OD010440RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BB/V007572/1RCUK | Biotechnology and Biological Sciences Research Council (BBSRC) BB/V015648/1Royal Society RSWF\R1\180008
6 · The paper itself

Abstract

The BH3-only protein EGL-1 is the key activator of apoptosis during C. elegans development. EGL-1 protein is thought to be synthesized predominantly in cells programmed to die and to localize to mitochondria. We used CRISPR-Cas-mediated modification of the egl-1 locus to add the coding sequence for the monomeric StayGold fluorescent protein or 18 copies of the SunTag peptide to the endogenous open reading frame. We found that tagged EGL-1 protein colocalizes with mitochondria in vivo and that mitochondrial localization is dependent on the anti-apoptotic BCL-2-like protein CED-9. Consistent with the presence of egl-1 mRNA in cells programmed to die as well as their progenitor cells ('mother' cells), EGL-1 protein is detected in both types of cells in vivo. Furthermore, real time imaging reveals that EGL-1 protein rapidly disappears from the mother cell prior to its division and that EGL-1 protein rapidly reappears specifically in the daughter cell programmed to die. Our results demonstrate CED-9 BCL-2-dependent mitochondrial localization of EGL-1 BH3-only protein and dynamic control of EGL-1 protein synthesis and degradation. Furthermore, we have identified additional levels of control of egl-1 BH3-only function that expand our understanding of apoptosis activation in vivo.

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsMitochondriaProto-Oncogene Proteins c-bcl-2Repressor ProteinsAnimalsApoptosisProteolysisCaenorhabditis elegans ProteinsCed-9 protein, C elegansEGL-1 protein, C elegansProto-Oncogene Proteins c-bcl-2Repressor Proteins

Identifiers

PMID41673492
PMCPMC13545084

What Socratic holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.