Evidence map›Paper›PMID 41677256›Full record

ArticlemBio2026

Involvement of L polymerase and heat shock proteins in the biogenesis of viral circular RNAs derived from respiratory syncytial virus.

Mingzhen Lin, Shanshan Miao, Xinru Yang, Yezhenghong Qiu, Zesen Mai, Yao Xiao, Mengyuan Xie, Yulong Luo, Caiqi Ma, Jifang Liu and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mBio, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Mingzhen Lin *Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Shanshan Miao *Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Xinru Yang *Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Yezhenghong Qiu *Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Zesen MaiKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Yao XiaoKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Mengyuan XieKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Yulong LuoKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Caiqi MaKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Jifang LiuKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.
Zhaoyu LiuKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.ORCID 0009-0007-5143-3585
Wenxia YaoKey Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, GuangDong Engineering Technology Research Center of Biological Targeting Diagnosis, Therapy and Rehabilitation, State Key Laboratory of Respiratory Disease, The Fifth Affiliated Hospital, Guangzhou Medical University, The Fifth Clinical College of Guangzhou Medical University, Guangzhou, China.ORCID 0000-0003-1677-9613

Funding

National Natural Science Foundation of China 32270153Open Project of State Key Laboratory of Respiratory Disease SKLRD-OP-202323the Science and Technology Program of Guangzhou 2024A03J0077the Science and Technology Program of Guangzhou 2024A04J8217
6 · The paper itself

Abstract

Circular RNAs (circRNAs) are new members of noncoding RNAs that have gained increased attention, and reports on viral circRNAs and their functions continue to emerge in recent years. However, the mechanisms underlying the biogenesis of viral circRNAs, especially those produced by RNA viruses, remain largely unexplored. Our recent research systematically identified and characterized viral circRNAs induced by respiratory syncytial virus (RSV), a human RNA virus for which no effective treatments exist. In this study, we further investigated the mechanism underlying the production of RSV circRNAs. We found that the viral L polymerase and the cellular HSP70 and HSP90 proteins associate with viral circRNAs through proteomic analysis of circRNA pulled-down lysates. These interactions were further confirmed using RNA-binding protein immunoprecipitation assays. We also observed that RSV circRNAs colocalize with the L polymerase and HSP70/90 proteins within the synthesis site, inclusion bodies (IBs). Moreover, both the viral L protein and the cellular HSP70 and HSP90 proteins modulate the production of viral circRNAs. Finally, the flanking AT/TA sequences of RSV circRNAs serve as

Indexed as

Heat-Shock ProteinsHSP70 Heat-Shock ProteinsHSP90 Heat-Shock ProteinsRespiratory Syncytial Virus, HumanRNA, CircularRNA-Dependent RNA PolymeraseRNA, ViralViral ProteinsCell LineHost-Pathogen InteractionsHumansHeat-Shock ProteinsHSP70 Heat-Shock ProteinsHSP90 Heat-Shock ProteinsRNA, CircularRNA-Dependent RNA PolymeraseRNA, ViralViral ProteinsbiogenesisHSP70HSP90inclusion bodiesL polymeraseRSVviral circRNAs

Identifiers

PMID41677256
PMCPMC12977508

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.