Evidence map›Paper›PMID 41677943›Full record

ArticleTropical animal health and production2026

Effect of humic acid supplementation on lamb gastrointestinal health and performance.

Diógenes Adriano Duarte Santana, Francieli Rolinski, Leticia Graziela Trombetta, Rafaela Maria Debastiani Göhringer, Tharini Xavier Accioly Kaled, Caroline Ramos Dos Santos, Saulo Henrique Weber, Cristina Santos Sotomaior, Rüdiger Daniel Ollhoff

Abstract read
In one paragraph

Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Diógenes Adriano Duarte SantanaGraduate Program in Animal Science, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Francieli RolinskiVeterinary Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Leticia Graziela TrombettaVeterinary Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Rafaela Maria Debastiani GöhringerVeterinary Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Tharini Xavier Accioly KaledVeterinary Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Caroline Ramos Dos SantosVeterinary Medicine, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Saulo Henrique WeberGraduate Program in Animal Science, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Cristina Santos SotomaiorGraduate Program in Animal Science, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil.
Rüdiger Daniel OllhoffGraduate Program in Animal Science, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil. daniel.ollhoff@pucpr.br.ORCID http://orcid.org/0000-0002-7972-9472

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gastrointestinal infections significantly impact sheep health and productivity, with diarrhea and parasites particularly threatening lambs. To address these challenges, feed additives offer solutions to enhance animal health and performance. This study aimed to investigate the effects of humic acids (HA) on the gastrointestinal tract and growth of lambs. Forty 1/2 Hampshire Down weaned lambs (aged 100.9 ± 7.1 days; average weight 24.4 ± 3.9 kg) were randomly allocated to two groups (20 each), balanced for weight and sex. The control group (CG) received a basal diet without HA supplementation, while the treatment group (TG) was fed a basal diet plus commercial HA at 500 mg/kg body weight/day for 56 days. Every two weeks, lambs underwent hematological, biochemical, parasitological, immunological, fecal consistency, and performance analyses. After the experimental period, 10 lambs per group were slaughtered. Histological analyses included measuring abomasal mucosa thickness and mucus production in the abomasum, duodenum, jejunum, and ileum. Tissues from the abomasum, duodenum, jejunum, ileum, and cecum were also used for leukocyte counts and scanning electron microscopy (SEM) evaluations, while parasitological analysis consisted of parasite recovery from the abomasum and small intestine. The TG exhibited improved fecal consistency, a decrease in CD8 lymphocytes in the abomasum and duodenum, and reduced platelet counts. In general, hematological, biochemical, parasitological, histological, and performance parameters did not differ significantly between groups. HA fragments were not able to be distinguished with SEM on gastrointestinal mucosa surface. Overall, HA supplementation positively impacted digestive health by mitigating gastrointestinal inflammation.

Indexed as

Gastrointestinal DiseasesGastrointestinal TractHumic SubstancesSheep DiseasesSheep, DomesticAnimal FeedAnimal Nutritional Physiological PhenomenaAnimalsDietDietary SupplementsFemaleMaleRandom AllocationSheepHumic SubstancesDiarrheaFeed additiveHaemonchus contortusHumic substancesImmune cells

Identifiers

PMID41677943
PMCPMC12901293

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.