Protocol to study the contribution of biomechanics in osteoarthritis development using a cartilage-on-chip model.

Elodie Faure, Veronique Chobaz, Diego de Haro, Elsa Lauwers, Nathalie Busso, Carlo Alberto Paggi, Sonia Nasi

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In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Elodie FaureDivision of Rheumatology, Department of Musculoskeletal Medicine, Lausanne University Hospital, 1010 Lausanne, Switzerland.

Veronique ChobazDivision of Rheumatology, Department of Musculoskeletal Medicine, Lausanne University Hospital, 1010 Lausanne, Switzerland.

Diego de HaroDivision of Infectious Diseases, Department of Medicine, Lausanne University Hospital, 1010 Lausanne, Switzerland.

Elsa LauwersChrn on-chip Biotechnologies B.V. (aka chiron), Maastricht 6229 GS, the Netherlands.

Nathalie BussoDivision of Rheumatology, Department of Musculoskeletal Medicine, Lausanne University Hospital, 1010 Lausanne, Switzerland.

Carlo Alberto PaggiChrn on-chip Biotechnologies B.V. (aka chiron), Maastricht 6229 GS, the Netherlands.

Sonia NasiDivision of Rheumatology, Department of Musculoskeletal Medicine, Lausanne University Hospital, 1010 Lausanne, Switzerland. Electronic address: sonia.nasi@chuv.ch.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol to study biomechanics and calcification in osteoarthritis using a cartilage-on-chip model. We describe steps for culturing human chondrocytes in 3D hydrogels and subjecting them to physiological loading. We then detail procedures for assessing spontaneous calcification via alizarin red S staining, gene expression using qPCR, protein expression by immunofluorescence, and protein secretome analysis through the Olink proximity extension assay. This protocol is adaptable and allows exploration of cartilage degeneration mechanisms under controlled conditions.

Indexed as

Cartilage, ArticularLab-On-A-Chip DevicesOsteoarthritisBiomechanical PhenomenaCell Culture TechniquesCells, CulturedChondrocytesHumansMicrophysiological SystemsBiotechnology and bioengineeringCell BiologyMolecular Biology

Identifiers

PMID41678339

DOI10.1016/j.xpro.2026.104365

PMCPMC12915146

What Socratic holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.