Evidence map›Paper›PMID 41679470›Full record

ArticleMolecules and cells2026

A brief guide to isolating primary hepatocytes from mice.

Jisoo Song, Garam An, Gwonhwa Song, Wei Ying, Whasun Lim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules and cells, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jisoo SongDepartment of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, Republic of Korea; Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Garam AnDepartment of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, Republic of Korea; Division of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, La Jolla 92093 CA, USA.
Gwonhwa SongDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
Wei YingDivision of Endocrinology and Metabolism, Department of Medicine, University of California, San Diego, La Jolla 92093 CA, USA. Electronic address: weying@health.ucsd.edu.
Whasun LimDepartment of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, Republic of Korea. Electronic address: wlim@skku.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The isolation of primary hepatocytes, which are liver parenchymal cells, is a valuable tool in biomedical research for exploring liver physiology and disease mechanisms. However, obtaining hepatocytes with high viability can be challenging owing to the technical complexity of the procedure and fragility of the cells. Here, we present an optimized protocol, along with key considerations, for isolating hepatocytes from mice with high yield and viability, thereby contributing to liver-related biomedical research.

Indexed as

Cell SeparationHepatocytesAnimalsCells, CulturedCell SurvivalLiverMiceCell cultureLiverLiver perfusionMousePrimary hepatocyte

Identifiers

PMID41679470
PMCPMC12954296

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.