Evidence map›Paper›PMID 41680753›Full record

ArticleBMC biology2026

Stretching inhibits tumor growth in MMTV-PYMT via a direct mechanical effect.

Lisbeth Berrueta, Chandra Bhan, Yi He, Rebecca D Thompson, Oscar Safairad, Andrew D Doyle, Paola Pérez, Daniel Martin, Mazen Mezher, Alexander Cartagena-Rivera and 2 more

Abstract read
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In one paragraph

Article in BMC biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lisbeth Berrueta *Connective Tissue Section, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, 20892, USA. Lisbeth.berruetacarrillo@nih.gov.ORCID 0000-0002-5674-6448
Chandra Bhan *Connective Tissue Section, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, 20892, USA.ORCID 0000-0001-6197-3285
Yi HeConnective Tissue Section, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, 20892, USA.ORCID 0000-0002-9503-0565
Rebecca D ThompsonConnective Tissue Section, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, 20892, USA.
Oscar SafairadConnective Tissue Section, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, 20892, USA.
Andrew D DoyleLaboratory of Cell and Developmental Biology, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, 20892, USA.ORCID 0000-0003-4150-5155
Paola PérezSalivary Disorders Unit, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0001-8294-0466
Daniel MartinGenomics and Computational Biology Core, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, USA.ORCID 0000-0002-8880-9087
Mazen MezherSection On Mechanobiology, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20892, USA.
Alexander Cartagena-RiveraSection On Mechanobiology, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, 20892, USA.ORCID 0000-0001-6227-2499
Gary J BadgerDepartment of Medical Biostatistics, University of Vermont, Burlington, VT, 05405, USA.
Helene M LangevinConnective Tissue Section, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, 20892, USA.ORCID 0000-0002-3142-1671

Funding

NIH Intramural Research Program of the National Institutes of Health
6 · The paper itself

Abstract

backgroundTumor-associated architecture and emerging mechanical properties (forces, pressure, tension, stiffness) affect the growth and invasiveness of cancer cells. Collagen fibers aligned perpendicular to the boundaries of tumors promote local tumor invasiveness in mouse mammary tumor models and are associated with a poor prognosis in human breast cancer. Our previous study revealed that daily gentle stretching (~ 25% strain) for 10 min reduced the growth of P53/PTEN - / - orthotopic mouse mammary tumors by 50%.

resultsIn this study, we further investigated the mechanism of stretching in a more aggressive MMTV-PYMT (mammary tumor virus- polyomavirus middle T antigen) tumor model in vivo and in vitro and analyzed its impact on collagen reorganization at both the tumor-stromal interface and the tumor microenvironment composition at single-cell level. Stretching reduced the average tumor size by 30-50% in orthotopic (Active and Passive Stretch) and transgenic (Passive Stretch) models. In the orthotopic model, the ratio of parallel vs. perpendicular collagen fibers relative to the tumor boundary was greater in the Stretch group compared with the No Stretch group. Finally, stretching reduced the cell migration of collagen-embedded tumor spheroids in vitro.

conclusionsThese results show that short-duration, moderate-amplitude stretching reduces tumor growth in several different animal models. We also provide evidence that this beneficial effect may be a direct mechanical effect on local matrix properties and tumor cell invasiveness.

Indexed as

Mammary Neoplasms, ExperimentalMammary Tumor Virus, MouseAnimalsCell Line, TumorCollagenFemaleMiceTumor MicroenvironmentCollagenBreast cancerExtracellular matrixMechanobiologyMMTV-PYMTStretching

Identifiers

PMID41680753
PMCPMC12997666

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.