Evidence map›Paper›PMID 41685932›Full record

ArticleMicrobiology spectrum2026

Development of an aptamer-based rapid diagnostic assay for

Abebe M Aga, Demise Mulugeta, Abera Motuma, Atsbeha Gebreegziabxier Weldemariam, Adugna Abdi Woldesemayat, Bilise Wakitole, Fanos Tadesse Woldemariyam, Chala Bashea, Musin Kelel, Zinash Teferi and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Abebe M AgaArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.ORCID 0000-0003-2268-7707
Demise MulugetaArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Abera MotumaArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Atsbeha Gebreegziabxier WeldemariamEthiopian Public Health Institute, Integrated Pathogen Genomics and Bioinformatics Facility for Surveillance and Research, Addis Ababa, Ethiopia.
Adugna Abdi WoldesemayatDepartment of Biotechnology and Bioprocess Centre of Excellence, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia.
Bilise WakitoleArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Fanos Tadesse WoldemariyamArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Chala BasheaEthiopian Public Health Institute, Integrated Pathogen Genomics and Bioinformatics Facility for Surveillance and Research, Addis Ababa, Ethiopia.
Musin KelelDepartment of Biotechnology and Bioprocess Centre of Excellence, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia.
Zinash TeferiArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Gemechu Chala HunderraArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Rajiha AbubekerEthiopian Public Health Institute, Integrated Pathogen Genomics and Bioinformatics Facility for Surveillance and Research, Addis Ababa, Ethiopia.
Gebremedhin GebremicaelEthiopian Public Health Institute, Integrated Pathogen Genomics and Bioinformatics Facility for Surveillance and Research, Addis Ababa, Ethiopia.
Abraham AliEthiopian Public Health Institute, Integrated Pathogen Genomics and Bioinformatics Facility for Surveillance and Research, Addis Ababa, Ethiopia.
Gemechu Tadesse LetaEthiopian Public Health Institute, Integrated Pathogen Genomics and Bioinformatics Facility for Surveillance and Research, Addis Ababa, Ethiopia.
Natnael BerihunArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Shambel TadesseArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Serkadis OljiraArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Dereje NigussieArmauer Hansen Research Institute, Vaccines, Diagnostics and Medical Device R&D, Addis Ababa, Ethiopia.
Mesfin Tafesse GemedaDepartment of Biotechnology and Bioprocess Centre of Excellence, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia.ORCID 0000-0002-1496-2863

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid and accurate detection of IMPORTANCE: This study addresses a critical need for rapid, low-cost, and sensitive diagnostic tools for Salmonella detection, particularly in low-resource settings where conventional methods are often inaccessible or time-consuming. By developing an aptamer-based colorimetric assay using silver nanoparticles, the research offers a highly specific, sensitive, and affordable alternative to existing diagnostics. The use of DNA aptamers selected through Systematic Evolution of Ligands by Exponential Enrichment enables precise recognition of Salmonella surface markers, while the colorimetric readout allows for easy visual detection without the need for sophisticated instruments. The assay's sensitivity demonstrates its practical utility for early and field-level pathogen detection. Importantly, the incorporation of next-generation sequencing in aptamer characterization enhances the molecular understanding of aptamer-target interactions and supports future assay refinement for application in lateral flow assay. The findings support translating the platform into point-of-care formats, which help food safety monitoring and infectious disease control in developing regions.

Indexed as

Aptamers, NucleotideSalmonellaSalmonella entericaSalmonella InfectionsSELEX Aptamer TechniqueColorimetryHumansMetal NanoparticlesRapid Diagnostic TestsSensitivity and SpecificitySilverAptamers, NucleotideSilvercolorimetric assayDNA aptamernanoparticlespoint-of-care diagnosticsSalmonella detectionwhole-cell SELEX

Identifiers

PMID41685932
PMCPMC13055386

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.