Investigation of Carbonic Anhydrase Inhibition, Antioxidant Properties, and Selective Anticancer Activity of Methyl-Substituted Halogenated and Methoxy Conduritols.

Introduction: This study aimed to investigate the anticancer, potential antiepileptic agents, and antioxidant potentials of 10 methyl-substituted halogenated and methoxy conduritols, which had been previously synthesized and characterized. The presence of active functional groups within their structures suggested their potential as bioactive molecules. Both in vitro and in silico approaches were employed to assess their biological activities and therapeutic relevance. Methods: Anticancer activity was tested using the MCF-7 breast cancer cell line and the L929 fibroblast cell line, with IC Results: Among the synthesized derivatives, only compound 6 demonstrated notable anticancer activity, with an IC₅₀ of 20.22 Conclusions: The findings indicate that methyl-substituted halogenated and methoxy conduritols possess anticancer, potential antiepileptic agents, and antioxidant potentials. Their strong carbonic anhydrase inhibitory activities highlight their promise as potential therapeutic agents for epilepsy and glaucoma. Overall, these compounds demonstrate considerable potential as multifunctional bioactive molecules and represent promising candidates for further preclinical studies.