ArticleDrug testing and analysis2026
Identification of Metabolites of the Dietary Supplement Ingredient 5α-Hydroxy-Laxogenin.
Article in Drug testing and analysis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 1 synthesis or guideline pooled it.
- Natural products alleviate exercise-induced fatigue by modulating gut microbiota: a systematic review.Journal of the International Society of Sports Nutrition · 2026Pooled it
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Illegally added, undeclared dietary supplement ingredients represent one way of unintentional exposure to substances that are prohibited by the World Anti-Doping Agency (WADA). In addition to the risk of anti-doping rule violation, adulterated dietary supplements can also be a health risk for elite and recreational athletes. 5α-hydroxy-laxogenin is one of those illegal substances but is not prohibited yet. Though an in vitro bioassay showed androgen receptor activation, this androgenic effect could not be confirmed in the preclinical orchiectomized rat model, which might be due to a fast biotransformation into inactive metabolites. In the present study, we investigated the metabolism of 5α-hydroxy-laxogenin using three different approaches: in silico prediction using the GLORYx tool of NERRD, in vitro biotransformation using the human hepatocellular cell line HepG2 and the preclinical orchiectomized rat model, from which serum and hair samples were collected and investigated. Analyses were performed using high-performance liquid chromatography-high-resolution mass spectrometry (HPLC-HRMS). In silico metabolism predicted five different metabolites resulting from hydroxylation, reduction, or oxidation. The human HepG2 cells generated five metabolites resulting from reduction or hydroxylation and from a combination of both. Three metabolites generated in vitro and an additional mono-hydroxylated metabolite were detected in the serum of the rats treated daily subcutaneously with 5α-hydroxy-laxogenin for 2 weeks. Hair analysis points to incorporation of 5α-hydroxy-laxogenin, though showing a rather high contamination most likely by saliva or urine. In conclusion, we identified and characterized metabolites of 5α-hydroxy-laxogenin, for which, however, the exact modification sites need to be investigated in future.
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What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.