ArticleApplied biochemistry and biotechnology2026
Bioprocessing and Functional Characterization of Hibiscus Hamabo Root Culture Bioactives: Antioxidant and Moisturizing Effects with Surfactin-Enhanced Penetration in Artificial Skin Models.
Article in Applied biochemistry and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Plant tissue culture is a promising platform for producing metabolites with pharmaceutical, nutraceutical, and cosmetic potential. A bioprocess was developed to produce antioxidative and moisturizing bioactive compounds from Hibiscus hamabo (HHSZ) root cultures, and a surfactin-assisted nanoemulsion was optimized to facilitate their effective transdermal delivery. We established a method for culturing HHSZ root in a controlled bioreactor where various conditions such as temperature and pH could be regulated, and subsequently established an extraction process. The 3T3 Neutral Red Uptake (NRU) and a 2’,7’-dichlorofluorescin diacetate (DCFDA) assay confirmed that root extract is non-phototoxic and has a reactive oxygen species (ROS) scavenging effect, respectively. Additionally, the root extract increased hyaluronic acid (HA) synthesis by 70% compared to the untreated sample. Protein expression analysis revealed an upregulation of antioxidant-related factors, including heme oxygenase 1 (HO-1) and phospho-NFκB repressing factor (p-NRF2), as well as hyaluronan synthase 2 (HAS2), a key factor in skin hydration. Similarly, mRNA analysis showed increased expression of HMOX1, the gene of HO-1, in both HaCaT and NHF cells and HAS2 gene in HaCaT cells. These results suggest that HHSZ possesses strong antioxidant and moisturizing properties, evidenced with metabolite profiling. In addition, biosurfactant surfactin-assisted nanodispersion enhanced permeability of the bioactive metabolites through skin layer. This study confirmed potential of HHSZ root extract and its surfactin-assisted nanoemulsion for use in cosmetics and pharmaceutical products.
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