Analysis of the characteristics of microRNA expression profiles and reproductive regulation-mediated signalling pathways in testicular sertoli derived sEVs from Leizhou goats.

Yang Li, Xiaowen Zeng, Huimingda Bao, Yongnan Zheng, Jing Wang, Guangdong Hu, Kai Kang, Jiang Wu

Abstract read

In one paragraph

Article in Veterinary research communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Yang LiCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.

Xiaowen ZengCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.

Huimingda BaoCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.

Yongnan ZhengCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China.

Jing WangCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, China.

Guangdong HuCollege of Animal Science and Technology, Shihezi University, Shihezi, 832000, China.

Kai KangCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China. Kangk@gdou.edu.cn.

Jiang WuCollege of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, 524088, China. Wuj@gdou.edu.cn.

Funding

the Guangdong Ocean University Student Innovation Training Program S202410566029the Marine Biology Foundation of Zhanjiang Bureau of Science and Technology 2021E05028the Natural Science Foundation of Guangdong Province 2020A1515110362the Science and Technology Program of XPCC 2024DA006

6 · The paper itself

Abstract

This study focused on the tropical-adapted Leizhou black goat to analyse the miRNA expression profile characteristics of testicular Sertoli cells (SCs) and SC-derived small extracellular vesicles (SC-sEVs) and the potential signalling pathways involved in reproductive regulation. SCs and SC-sEVs were isolated and purified, and their characteristics were verified through morphology, particle size analysis, and identification of marker proteins. Illumina high-throughput sequencing was used to analyse the miRNA expression profile of SC-sEVs. Target genes of differentially expressed miRNAs were predicted using TargetScan (v7.0) and miRanda (v3.3a) and their biological functions were analysed via GO and KEGG pathway enrichment. The results indicated that the SCs and their sEVs were successfully isolated with high purity. Small RNA sequencing of sEVs (Illumina NovaSeq, n = 3) revealed 387 known miRNAs and 52 novel miRNAs. miRNAs with 19-nt lengths were predominant, and the top 20 most abundant miRNAs accounted for 61% of total reads. A total of 9176 target genes were significantly enriched in the Hippo (p = 1.3 × 10− 4), TGF-β (p = 3.9 × 10− 4), MAPK, JAK-STAT, Wnt, and cAMP signalling pathways. This study comprehensively characterised the miRNA expression profile of goat SC-sEVs and confirmed that the miRNAs enriched in SC-sEVs could regulate reproduction-related pathways through target genes, thereby contributing to the maintenance of the spermatogenic microenvironment.

Indexed as

Extracellular VesiclesGoatsSertoli CellsTestisAnimalsGene Expression ProfilingGene Expression RegulationMaleMicroRNAsReproductionSignal TransductionTranscriptomeMicroRNAsLeizhou goatMale reproductive signallingMiRNASertoli cell sEVSpermatogenesis regulation

Identifiers

PMID41712113

DOI10.3390/ijms252313126

What Socratic holds

Textmetadata

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.