Evidence map›Paper›PMID 41715892›Full record

ArticleRNA biology2026

Optimizing protocols for microRNA profiling of infant and toddler stool.

David A Armstrong, Shannon M Soucy, Meghan E Muse, Fred W Kolling, Heidi W Trask, Alexandra L Howell, Hannah E Laue, Anne G Hoen, Jiang Gui, Brock C Christensen and 3 more

Abstract read
In one paragraph

Article in RNA biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

David A ArmstrongDepartment of Dermatology, Dartmouth Health, Lebanon, NH, USA.ORCID 0000-0003-1748-2520
Shannon M SoucyGenomic Data Science Core, Center for Quantitative Biology (COBRE), Dartmouth College, Hanover NH, USA.ORCID 0000-0001-8672-316X
Meghan E MuseDepartment of Epidemiology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.ORCID 0000-0002-4775-2133
Fred W KollingDartmouth Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.ORCID 0000-0002-6178-9901
Heidi W TraskDartmouth Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
Alexandra L HowellResearch Service, V.A. Medical Center, White River Junction, VT, USA.ORCID 0000-0002-2362-2596
Hannah E LaueDepartment of Biostatistics and Epidemiology, University of Massachusetts Amherst School of Public Health and Health Sciences, Amherst, MA, USA.ORCID 0000-0002-2578-103X
Anne G HoenDepartment of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.ORCID 0000-0003-0035-8689
Jiang GuiDartmouth Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
Brock C ChristensenDartmouth Cancer Center, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.ORCID 0000-0003-3022-426X
Juliette C MadanDepartment of Epidemiology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.ORCID 0000-0003-0542-1368
Margaret R KaragasDepartment of Epidemiology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.ORCID 0000-0002-6398-7362
Caitlin G HoweDepartment of Epidemiology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.ORCID 0000-0002-4935-9298

Funding

Translational Engineering in Cancer (TEC)P30CA023108 · NCI · DARTMOUTH COLLEGE · PI Brock C Christensen · 1985 to 2026
$91.3M
Zhao - Proj 2P20GM130454 · NIGMS · DARTMOUTH COLLEGE · PI Li Song · 2019 to 2026
$27.2M
Relation Between In-utero Vitamin D and Immune Function in Early Childhood P20GM104416 · NIGMS · DARTMOUTH COLLEGE · PI PASSARELLI, MICHAEL N · 2013 to 2022
$23.7M
Pilot Project ProgramP30GM149408 · NIGMS · DARTMOUTH COLLEGE · PI Brock C Christensen · 2023 to 2026
$6.3M
NCI NIH HHS P30 CA023108NIGMS NIH HHS P20 GM104416NIGMS NIH HHS P20 GM130454NIGMS NIH HHS P30 GM149408
6 · The paper itself

Abstract

Despite growing interest in profiling microRNAs (miRNAs) in infant and toddler stool, no studies have compared protocols for preserving and extracting miRNAs from this specimen type. Three commercially available kits and four preservation methods were compared for their ability to yield high quality RNA from children <2 years of age (infant/toddler). Of the three RNA extraction kits compared, Zymo BIOMICs yielded the highest RNA Quality Number (RQN) (median (range) RQN 9.4 (5.7-10.0)). Of the four preservation methods tested, RNAlater and Zymo DNA/RNA Shield Faecal Collection Tubes yielded the highest two RQNs (median (range) RQN 9.8 (5.7-10.0) and 9.4 (5.4-10.0), respectively), which did not differ from each other ( Collecting stool from infants and toddlers <2 years of age in either RNAlater or Zymo DNA/RNA Shield Faecal Collection Tubes, when paired with RNA extraction using the Zymo BIOMICs extraction kit, yielded high-quality RNA with similar human miRNA profiles.

Indexed as

FecesGene Expression ProfilingMicroRNAsChild, PreschoolHumansInfantSpecimen HandlingMicroRNAsinfantMicroRNARNA extractionRNA preservationRNA qualitystooltoddler

Identifiers

PMID41715892
PMCPMC12962672

What Socratic holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.