Evidence map›Paper›PMID 41718886›Full record

ArticleMolecular and cellular biochemistry2026

Optimization of RNP-CRISPR for high-efficiency gene editing in mouse intestinal organoids.

Xiya Cao, Youyuan He, Haozhe Guo, Xiaoya Cao, Dan Zhang, Yuxuan Lai, Weihua Yang, Zixuan Ma, Xinghua Yu, Liren Wang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular biochemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiya CaoShanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Youyuan HeThe Third School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, Guangdong, China.
Haozhe GuoShanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Xiaoya CaoFengxian Central Hospital Affiliated to Southern Medical University, Shanghai, 201499, China.
Dan ZhangShanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University, Shanghai, 200241, China.
Yuxuan LaiThe Third School of Clinical Medicine, Southern Medical University, Guangzhou, 510515, Guangdong, China.
Weihua YangSchool of Medicine, Anhui University of Science & Technology, Huainan, 232001, Anhui, China.
Zixuan MaWLSA Shanghai academy, Shanghai, 200092, China.
Xinghua YuDepartment of Urology & Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, 310030, Zhejiang, China. yuxinghua@zju.edu.cn.
Liren WangShanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University, Shanghai, 200241, China. lrwang@bio.ecnu.edu.cn.
Dali LiShanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University, Shanghai, 200241, China. dlli@bio.ecnu.edu.cn.
Zhiyang ZengShanghai Frontiers Science Center of Genome Editing and Cell Therapy, Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University, Shanghai, 200241, China. zyzeng1991@163.com.

Funding

Innovation Program of Shanghai Municipal Education Commission 2019-01-07-00-05-E00054National Key Research and Development Program of China 2019YFA0110802National Natural Science Foundation of China No.32025023National Natural Science Foundation of China No.32200732Shanghai Municipal Commission for Science and Technology 20MC1920400Shanghai Municipal Commission for Science and Technology 22YF1437700
6 · The paper itself

Abstract

Mouse intestinal organoids are ideal models for investigating intestinal development and diseases. The full potential of these models hinges on the ability to precisely engineer their genome, yet traditional methods for CRISPR-based editing in 3D cultures often surfer from low efficiency, high cytotoxicity, and inconsistent post-editing differentiation, which limits their applications. Here, we developed an electroporation approach mediated by ribonucleoprotein (RNP)-CRISPR that achieves over 90% gene editing efficiency in mouse intestinal organoids. Using this optimized method, we generated APC-knockout organoids that exhibit Wnt pathway hyperactivation, demonstrated by R-spondin1-independent growth, increased nuclear β-catenin, and enhanced proliferation. Our method addresses a critical technical gap in murine organoid research, offering a scalable platform for intestinal disease modeling.

Indexed as

CRISPR-Cas SystemsGene EditingIntestinesOrganoidsRibonucleoproteinsAnimalsElectroporationMiceRibonucleoproteinsAPCCRISPR/Cas9ElectroporationGenome editingOrganoids

Identifiers

PMID41718886
PMCPMC13061793

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.