Article in Communications biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Lora L PlessDepartment of Medicine, Division of Infectious Diseases, University of Pittsburgh, Pittsburgh, PA, USA.
Kelin HeCenter for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA, USA.
Tianhan LingCenter for Biologic Imaging, University of Pittsburgh, Pittsburgh, PA, USA.
Jenesis KozelDepartment of Psychiatry, University of Pittsburgh, Pittsburgh, PA, USA.
Stephanie PuigDepartment of Psychiatry and Behavioral Sciences, University of Massachusetts Chan Medical School, Worcester, MA, USA.ORCID http://orcid.org/0000-0001-7200-1094
Benjamin R WilliamsDepartment of Psychiatry and Behavioral Sciences, University of Massachusetts Chan Medical School, Worcester, MA, USA.ORCID http://orcid.org/0009-0001-6548-518X
Mackenzie C GambleMolecular and Translational Medicine, Department of Medicine, Boston University School of Medicine, Boston, MA, USA.
Ryan W LoganDepartment of Psychiatry and Behavioral Sciences, University of Massachusetts Chan Medical School, Worcester, MA, USA. ryan.logan@umassmed.edu.ORCID http://orcid.org/0000-0001-8579-015X
A Confocal Fluorescence Microscopy Brain Data ArchiveR24MH114793 · NIMH · CARNEGIE-MELLON UNIVERSITY · PI ALEXANDER J ROPELEWSKI, Alan Michael Watson · 2017 to 2026
$10.1M
Novel roles of VGLUT in sex differences in dopamine neuron vulnerability to environmental toxicant-induced neurodegenerationR01ES034037 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ZACHARY FREYBERG · 2023 to 2026
$2.3M
Interinstitutional Program in Cell and Molecular Biology: A Graduate Training Path to Promote Traditional and Non-Traditional Professional OutcomesT32GM133353 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BRODSKY, JEFFREY L., MURRAY, SANDRA ANN · 2020 to 2024
$1.5M
Novel roles for the circadian transcription factor NPAS2 and striatal dopamine D3 receptor signaling in diurnal fentanyl craving and relapseR01DA061243 · NIDA · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI ZACHARY FREYBERG, Ryan W Logan · 2024 to 2026
$1.4M
Novel dopaminergic mechanisms of islet hormone secretion and antipsychotic drug-induced metabolic disturbancesR01DK124219 · NIDDK · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI FREYBERG, ZACHARY · 2021 to 2023
$1.2M
Ultra-fast high-resolution imaging of whole mouse brain for the study of drug addictionR21DA052419 · NIDA · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI FREYBERG, ZACHARY, LOGAN, RYAN W · 2021 to 2022
$448k
Request for Dell PowerEdge XE9680 8x H100 NVIDIA GPU serverS10OD038197 · OD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI WATSON, ALAN MICHAEL · 2025 to 2025
$325k
Dissecting the functional relevance of unique subpopulations of striatal dopamine receptors in opioid use disorderR36DA057972 · NIDA · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI KOZEL, JENESIS · 2023 to 2024
$108k
NIDA NIH HHS R01 DA061243NIDA NIH HHS R21 DA052419NIDA NIH HHS R36 DA057972NIDDK NIH HHS R01 DK124219NIEHS NIH HHS R01 ES034037NIGMS NIH HHS T32 GM133353NIH HHS S10 OD038197NIMH NIH HHS R24 MH114793U.S. Department of Defense (United States Department of Defense) PR210207U.S. Department of Health & Human Services | NIH | National Institute of Diabetes and Digestive and Kidney Diseases (National Institute of Diabetes & Digestive & Kidney Diseases) R01DK124219U.S. Department of Health & Human Services | NIH | National Institute of Environmental Health Sciences (NIEHS) R01ES034037U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) T32GM133353U.S. Department of Health & Human Services | NIH | National Institute of Mental Health (NIMH) R24MH114793U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse (NIDA) R01DA061243U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse (NIDA) R21DA052419U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse (NIDA) R36DA057972U.S. Department of Health & Human Services | NIH | NIH Office of the Director (OD) S10OD038197
6 · The paper itself
Abstract
The field of neuroscience has been transformed by recent advances in spatial mapping of neuronal activity across whole cleared brains. Rapid adoption of these techniques requires computational workflows that can facilitate experiments comparing multiple conditions across large cohorts of individuals. We therefore developed a scalable approach for anatomical mapping of c-Fos positive cells in whole brain and applied it to map the response to the prototypic opioid, morphine. The analysis revealed distinct patterns of morphine-induced regional brain activation across both time and sex. These results support the multi-wave model of opioid-induced brain activation. Male mice displayed higher c-Fos expression than females in several key brain regions including nucleus accumbens, central amygdalar nucleus, ventral pallidum, prelimbic area, anterior cingulate area, and olfactory tubercle. Overall, this workflow can be applied to not only examine spatiotemporal actions of drugs of abuse on neuronal activity across the brain, but also mapping neuronal activity more generally.
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.
Brain-wide mapping reveals temporal and sexually dimorphic opioid actions. · full record | Socratic