Evidence map›Paper›PMID 41725354›Full record

ArticleDevelopment (Cambridge, England)2026

The emergence of multiple testicular cell lineages in human stem cell-derived testis-like organoids.

Svenja Pachernegg, Gorjana Robevska, Lucas G A Ferreira, Natalie Charitakis, Jinchao Gu, Jan Terhag, Eliza Martin, Denis Bienroth, Jocelyn van den Bergen, Sean B Wilson and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Svenja PacherneggReproductive Development, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.ORCID 0009-0003-9116-6064
Gorjana RobevskaReproductive Development, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Lucas G A FerreiraReproductive Development, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Natalie CharitakisTranscriptomics and Bioinformatics, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Jinchao GuDepartment of Neuroscience, School of Translational Medicine, Monash University, Melbourne, VIC 3004, Australia.
Jan TerhagResearch Support Operations, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Eliza MartinReproductive Development, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Denis BienrothTranscriptomics and Bioinformatics, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Jocelyn van den BergenReproductive Development, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Sean B WilsonNovo Nordisk Foundation Centre for Stem Cell Medicine, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Fernando J RosselloTranscriptomics and Bioinformatics, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Ben RolloDepartment of Neuroscience, School of Translational Medicine, Monash University, Melbourne, VIC 3004, Australia.
Melissa H LittleNovo Nordisk Foundation Centre for Stem Cell Medicine, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Mirana RamialisonDepartment of Paediatrics, University of Melbourne, Parkville, VIC 3010, Australia.
Andrew H SinclairReproductive Development, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.
Katie L AyersReproductive Development, Murdoch Children's Research Institute, Parkville, VIC 3052, Australia.ORCID 0000-0002-6840-3186

Funding

Cybec FoundationCybec Foundation (AU)National Health and Medical Research CouncilNational Health and Medical Research Council (AU) APP2012250National Stem Cell Foundation of AustraliaNovo Nordisk Foundation (DK) NNF21CC0073729University of Melbourne
6 · The paper itself

Abstract

Reproductive development is a complex process orchestrated by precise gene expression and cellular interactions. Disruption to this process can result in differences of sex development (DSDs) which occur in approximately 1-2% of live births. We have previously developed a protocol to differentiate human induced pluripotent stem cells (hiPSCs) into testis-like organoids. In this study, we performed bulk and single-cell RNA-sequencing on these organoids to investigate their transcriptional landscape. Transcriptomic analysis revealed six distinct cell clusters expressing markers associated with bipotential, early Sertoli and testicular interstitial cells. These findings provide the first comprehensive transcriptional profile of hiPSC-derived testis-like organoids. Additionally, to address the limited emergence of mature cell types, we generated an inducible NR5A1/SF1 hiPSC line, which successfully triggered the upregulation of Leydig cell markers and additional Sertoli markers upon overexpression. Our findings show that our testis-like organoids are a valuable model system for studying DSDs in vitro.

Indexed as

Cell LineageInduced Pluripotent Stem CellsOrganoidsTestisCell DifferentiationCell LineGene Expression ProfilingHumansLeydig CellsMaleSertoli CellsDifferences of sex developmentDSDGonadal developmentInducible SF1 cell lineTestis developmentTranscriptomics

Identifiers

PMID41725354
PMCPMC12989076

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.