Evidence mapPaperPMID 41729927Full record

Trial reportPloS one2026

The effects of high-intensity interval training on NLRP3 inflammasome and monocyte chemokine receptors in individuals with obesity.

Ana Luíza Pereira Assunção Silveira, Daniela Alves de Abreu, Amanda de Lima Santos Musto, Luiz Henrique da Silva Nali, Jônatas Bussador do Amaral, André Luis Lacerda Bachi, Lucas Melo Neves, Saulo Gil, Fernando Mateus Santos, Jefferson Russo Victor and 2 more

Abstract readRandomized Controlled Trial
In one paragraph

Trial report in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ana Luíza Pereira Assunção SilveiraPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Daniela Alves de AbreuPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Amanda de Lima Santos MustoPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Luiz Henrique da Silva NaliPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Jônatas Bussador do AmaralENT Research Lab. Department of Otorhinolaryngology -Head and Neck Surgery, Federal University of Sao Paulo, São Paulo, Brazil.
André Luis Lacerda BachiPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Lucas Melo NevesPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Saulo GilPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Fernando Mateus SantosPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Jefferson Russo VictorPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Marina Tiemi ShioPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.
Carolina Nunes FrançaPost Graduation Program in Health Sciences, Santo Amaro University, São Paulo, Brazil.ORCID https://orcid.org/0000-0002-4167-4293

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background/ Objectives: Obesity is a chronic disease that promotes increased cytokine production mediated by activating a complex of intracellular proteins known as the inflammasome, mainly NLRP3. The modulation of the NLRP3 inflammasome and monocyte chemokine receptors induced by high-intensity interval training (HIIT) in individuals with obesity is still poorly understood. This training is characterized by a period of high-intensity stimulation followed by a recovery period. The main objective of the current study was to investigate possible modulation of the NLRP3 inflammasome and monocyte chemokine receptors in individuals with obesity following HIIT. SUBJECTS/

methodsIn this randomized trial, we included individuals with obesity (n = 109), who have not exercised in the last six months, of both sexes, aged between 18 and 60 years.

interventionsParticipants were divided into a trained group, which performed three weekly HIIT sessions over eight weeks, and an untrained group (control). The NLRP3 inflammasome and its components, as well as monocyte chemokine receptors expression were analyzed before and after training period by real-time PCR.

resultsWhereas NLRP3 expression were unchanged (p = 0.08), after HIIT there was a decrease in Caspase-1 (CASP-1) expression in contrast to the increase of ASC expression as compared to baseline values (p = 0.04 and p < 0.0001, respectively). Additionally, lower IL-6 expression (p < 0.0001), with no differences in IL-1 β and IL-18 expressions were found after HIIT. Besides, HIIT led to an increase in the expression of CCR2 and CCR5 and a decrease in CX3CR1 expression (p < 0.0001, p = 0.001 and p = 0.007, respectively) compared to baseline. Differentially expressed genes interaction analysis [CASP-1, ASC (PYCARD), IL-6, CCR2, CXC3CR1 and CCR5] revealed interaction with NLRP3 nflammasome genes.

conclusionsEight weeks of HIIT can modulate some components involved in the NLRP3 inflammasome, as well as monocyte chemokine receptors, in individuals with obesity, suggesting an improvement in the control of inflammatory status.

trial registrationBrazilian Registry of Clinical Trials - ReBEC number RBR-8vfxfqd).

Indexed as

High-Intensity Interval TrainingInflammasomesMonocytesNLR Family, Pyrin Domain-Containing 3 ProteinObesityReceptors, ChemokineAdolescentAdultCaspase 1FemaleHumansInterleukin-18Interleukin-1betaInterleukin-6MaleMiddle AgedCaspase 1InflammasomesInterleukin-18Interleukin-1betaInterleukin-6NLR Family, Pyrin Domain-Containing 3 ProteinNLRP3 protein, humanReceptors, CCR2Receptors, CCR5Receptors, Chemokine

Identifiers

PMID41729927
PMCPMC12928487

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.